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Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function

Hsp70 binding protein 1 (HspBP1) and Bcl2-associated athanogene 1 (BAG-1), the functional orthologous nucleotide exchange factors of the heat shock protein 70 kilodalton (Hsc70/Hsp70) chaperones, catalyze the release of ADP from Hsp70 while inducing different conformational changes of the ATPase dom...

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Autores principales: Knapp, Regina T., Wong, Michael J. H., Kollmannsberger, Lorenz K., Gassen, Nils C., Kretzschmar, Anja, Zschocke, Jürgen, Hafner, Kathrin, Young, Jason C., Rein, Theo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891853/
https://www.ncbi.nlm.nih.gov/pubmed/24454860
http://dx.doi.org/10.1371/journal.pone.0085415
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author Knapp, Regina T.
Wong, Michael J. H.
Kollmannsberger, Lorenz K.
Gassen, Nils C.
Kretzschmar, Anja
Zschocke, Jürgen
Hafner, Kathrin
Young, Jason C.
Rein, Theo
author_facet Knapp, Regina T.
Wong, Michael J. H.
Kollmannsberger, Lorenz K.
Gassen, Nils C.
Kretzschmar, Anja
Zschocke, Jürgen
Hafner, Kathrin
Young, Jason C.
Rein, Theo
author_sort Knapp, Regina T.
collection PubMed
description Hsp70 binding protein 1 (HspBP1) and Bcl2-associated athanogene 1 (BAG-1), the functional orthologous nucleotide exchange factors of the heat shock protein 70 kilodalton (Hsc70/Hsp70) chaperones, catalyze the release of ADP from Hsp70 while inducing different conformational changes of the ATPase domain of Hsp70. An appropriate exchange rate of ADP/ATP is crucial for chaperone-dependent protein folding processes. Among Hsp70 client proteins are steroid receptors such as the glucocorticoid receptor (GR), the mineralocorticoid receptor (MR), and the androgen receptor (AR). BAG-1 diversely affects steroid receptor activity, while to date the influence of HspBP1 on steroid receptor function is mostly unknown. Here, we compared the influence of HspBP1 and BAG-1M on Hsp70-mediated steroid receptor folding complexes and steroid receptor activity. Coimmunoprecipitation studies indicated preferential binding of Hsp40 and the steroid receptors to BAG-1M as compared to HspBP1. Furthermore, Hsp70 binding to the ligand-binding domain of GR was reduced in the presence of HspBP1 but not in the presence of BAG-1M as shown by pull-down assays. Reporter gene experiments revealed an inhibitory effect on GR, MR, and AR at a wide range of HspBP1 protein levels and at hormone concentrations at or approaching saturation. BAG-1M exhibited a transition from stimulatory effects at low BAG-1M levels to inhibitory effects at higher BAG-1M levels. Overall, BAG-1M and HspBP1 had differential impacts on the dynamic composition of steroid receptor folding complexes and on receptor function with important implications for steroid receptor physiology.
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spelling pubmed-38918532014-01-21 Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function Knapp, Regina T. Wong, Michael J. H. Kollmannsberger, Lorenz K. Gassen, Nils C. Kretzschmar, Anja Zschocke, Jürgen Hafner, Kathrin Young, Jason C. Rein, Theo PLoS One Research Article Hsp70 binding protein 1 (HspBP1) and Bcl2-associated athanogene 1 (BAG-1), the functional orthologous nucleotide exchange factors of the heat shock protein 70 kilodalton (Hsc70/Hsp70) chaperones, catalyze the release of ADP from Hsp70 while inducing different conformational changes of the ATPase domain of Hsp70. An appropriate exchange rate of ADP/ATP is crucial for chaperone-dependent protein folding processes. Among Hsp70 client proteins are steroid receptors such as the glucocorticoid receptor (GR), the mineralocorticoid receptor (MR), and the androgen receptor (AR). BAG-1 diversely affects steroid receptor activity, while to date the influence of HspBP1 on steroid receptor function is mostly unknown. Here, we compared the influence of HspBP1 and BAG-1M on Hsp70-mediated steroid receptor folding complexes and steroid receptor activity. Coimmunoprecipitation studies indicated preferential binding of Hsp40 and the steroid receptors to BAG-1M as compared to HspBP1. Furthermore, Hsp70 binding to the ligand-binding domain of GR was reduced in the presence of HspBP1 but not in the presence of BAG-1M as shown by pull-down assays. Reporter gene experiments revealed an inhibitory effect on GR, MR, and AR at a wide range of HspBP1 protein levels and at hormone concentrations at or approaching saturation. BAG-1M exhibited a transition from stimulatory effects at low BAG-1M levels to inhibitory effects at higher BAG-1M levels. Overall, BAG-1M and HspBP1 had differential impacts on the dynamic composition of steroid receptor folding complexes and on receptor function with important implications for steroid receptor physiology. Public Library of Science 2014-01-14 /pmc/articles/PMC3891853/ /pubmed/24454860 http://dx.doi.org/10.1371/journal.pone.0085415 Text en © 2014 Knapp et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Knapp, Regina T.
Wong, Michael J. H.
Kollmannsberger, Lorenz K.
Gassen, Nils C.
Kretzschmar, Anja
Zschocke, Jürgen
Hafner, Kathrin
Young, Jason C.
Rein, Theo
Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title_full Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title_fullStr Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title_full_unstemmed Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title_short Hsp70 Cochaperones HspBP1 and BAG-1M Differentially Regulate Steroid Hormone Receptor Function
title_sort hsp70 cochaperones hspbp1 and bag-1m differentially regulate steroid hormone receptor function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891853/
https://www.ncbi.nlm.nih.gov/pubmed/24454860
http://dx.doi.org/10.1371/journal.pone.0085415
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