Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing

AIM: Rapid detection of H.pylori strains by PCR-Sequencing. METHODS: 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI –BLAST software. RESULTS: The PCR...

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Detalles Bibliográficos
Autores principales: Banerjee, Hirendra nath, Gramby, Monique, Hawkins, Zack
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891930/
https://www.ncbi.nlm.nih.gov/pubmed/24443707
http://dx.doi.org/10.4172/2155-9821.1000105e
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author Banerjee, Hirendra nath
Gramby, Monique
Hawkins, Zack
author_facet Banerjee, Hirendra nath
Gramby, Monique
Hawkins, Zack
author_sort Banerjee, Hirendra nath
collection PubMed
description AIM: Rapid detection of H.pylori strains by PCR-Sequencing. METHODS: 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI –BLAST software. RESULTS: The PCR –Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H.pylori strain#26695. CONCLUSION: The pathogenicity of H.pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen.
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spelling pubmed-38919302014-01-15 Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing Banerjee, Hirendra nath Gramby, Monique Hawkins, Zack J Bioprocess Biotech Article AIM: Rapid detection of H.pylori strains by PCR-Sequencing. METHODS: 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI –BLAST software. RESULTS: The PCR –Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be of H.pylori strain#26695. CONCLUSION: The pathogenicity of H.pylori depends on the strain of the bacteria, PCR-Sequencing and analysis of the sequence data by BLAST can be a very quick and useful diagnostic method of the pathogen. 2011-10-19 /pmc/articles/PMC3891930/ /pubmed/24443707 http://dx.doi.org/10.4172/2155-9821.1000105e Text en © 2011 Banerjee HN, et al. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Banerjee, Hirendra nath
Gramby, Monique
Hawkins, Zack
Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title_full Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title_fullStr Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title_full_unstemmed Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title_short Molecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
title_sort molecular diagnosis of helicobacter pylori strain by 16s rdna pcr amplification and direct sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891930/
https://www.ncbi.nlm.nih.gov/pubmed/24443707
http://dx.doi.org/10.4172/2155-9821.1000105e
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