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Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients

Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients. The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-r...

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Autores principales: Kratz, Ewa M., Ferens-Sieczkowska, Mirosława, Faundez, Ricardo, Kątnik-Prastowska, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892108/
https://www.ncbi.nlm.nih.gov/pubmed/24057866
http://dx.doi.org/10.1007/s10719-013-9501-y
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author Kratz, Ewa M.
Ferens-Sieczkowska, Mirosława
Faundez, Ricardo
Kątnik-Prastowska, Iwona
author_facet Kratz, Ewa M.
Ferens-Sieczkowska, Mirosława
Faundez, Ricardo
Kątnik-Prastowska, Iwona
author_sort Kratz, Ewa M.
collection PubMed
description Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients. The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively. However, a correlation between IgG core fucosylation and IgG concentration (r = −0.52; p < 0.0003) was observed. The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002). In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa. The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group. In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands. Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively). The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
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spelling pubmed-38921082014-01-21 Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients Kratz, Ewa M. Ferens-Sieczkowska, Mirosława Faundez, Ricardo Kątnik-Prastowska, Iwona Glycoconj J Article Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients. The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively. However, a correlation between IgG core fucosylation and IgG concentration (r = −0.52; p < 0.0003) was observed. The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002). In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa. The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group. In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands. Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively). The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation. Springer US 2013-09-22 2014 /pmc/articles/PMC3892108/ /pubmed/24057866 http://dx.doi.org/10.1007/s10719-013-9501-y Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Kratz, Ewa M.
Ferens-Sieczkowska, Mirosława
Faundez, Ricardo
Kątnik-Prastowska, Iwona
Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title_full Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title_fullStr Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title_full_unstemmed Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title_short Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients
title_sort changes in fucosylation of human seminal igg and secretory component of iga in leukocytospermic patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892108/
https://www.ncbi.nlm.nih.gov/pubmed/24057866
http://dx.doi.org/10.1007/s10719-013-9501-y
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