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Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578
BACKGROUND: Klebsiella pneumoniae is a leading cause of hospital-acquired urinary tract infections and pneumonia worldwide, and is responsible for many cases of pyogenic liver abscess among diabetic patients in Asia. A defining characteristic of this pathogen is the presence of a thick, exterior cap...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892127/ https://www.ncbi.nlm.nih.gov/pubmed/24398052 http://dx.doi.org/10.1186/1756-0500-7-13 |
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author | Huang, Tzu-Wen Lam, Irene Chang, Hwan-You Tsai, Shih-Feng Palsson, Bernhard O Charusanti, Pep |
author_facet | Huang, Tzu-Wen Lam, Irene Chang, Hwan-You Tsai, Shih-Feng Palsson, Bernhard O Charusanti, Pep |
author_sort | Huang, Tzu-Wen |
collection | PubMed |
description | BACKGROUND: Klebsiella pneumoniae is a leading cause of hospital-acquired urinary tract infections and pneumonia worldwide, and is responsible for many cases of pyogenic liver abscess among diabetic patients in Asia. A defining characteristic of this pathogen is the presence of a thick, exterior capsule that has been reported to play a role in biofilm formation and to protect the organism from threats such antibiotics and host immune challenge. FINDINGS: We constructed two knockout mutants of K. pneumoniae to investigate how perturbations to capsule biosynthesis alter the cellular phenotype. In the first mutant, we deleted the entire gene cluster responsible for biosynthesis of the extracellular polysaccharide capsule. In the second mutant, we deleted the capsule export subsystem within this cluster. We find that both knockout mutants have lower amounts of capsule but produce greater amounts of biofilm. Moreover, one of the two mutants abolishes fimbriae expression as well. CONCLUSIONS: These results are expected to provide insight into the interaction between capsule biosynthesis, biofilm formation, and fimbriae expression in this organism. |
format | Online Article Text |
id | pubmed-3892127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38921272014-01-16 Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 Huang, Tzu-Wen Lam, Irene Chang, Hwan-You Tsai, Shih-Feng Palsson, Bernhard O Charusanti, Pep BMC Res Notes Short Report BACKGROUND: Klebsiella pneumoniae is a leading cause of hospital-acquired urinary tract infections and pneumonia worldwide, and is responsible for many cases of pyogenic liver abscess among diabetic patients in Asia. A defining characteristic of this pathogen is the presence of a thick, exterior capsule that has been reported to play a role in biofilm formation and to protect the organism from threats such antibiotics and host immune challenge. FINDINGS: We constructed two knockout mutants of K. pneumoniae to investigate how perturbations to capsule biosynthesis alter the cellular phenotype. In the first mutant, we deleted the entire gene cluster responsible for biosynthesis of the extracellular polysaccharide capsule. In the second mutant, we deleted the capsule export subsystem within this cluster. We find that both knockout mutants have lower amounts of capsule but produce greater amounts of biofilm. Moreover, one of the two mutants abolishes fimbriae expression as well. CONCLUSIONS: These results are expected to provide insight into the interaction between capsule biosynthesis, biofilm formation, and fimbriae expression in this organism. BioMed Central 2014-01-08 /pmc/articles/PMC3892127/ /pubmed/24398052 http://dx.doi.org/10.1186/1756-0500-7-13 Text en Copyright © 2014 Huang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Huang, Tzu-Wen Lam, Irene Chang, Hwan-You Tsai, Shih-Feng Palsson, Bernhard O Charusanti, Pep Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title | Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title_full | Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title_fullStr | Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title_full_unstemmed | Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title_short | Capsule deletion via a λ-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578 |
title_sort | capsule deletion via a λ-red knockout system perturbs biofilm formation and fimbriae expression in klebsiella pneumoniae mgh 78578 |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892127/ https://www.ncbi.nlm.nih.gov/pubmed/24398052 http://dx.doi.org/10.1186/1756-0500-7-13 |
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