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Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis

Lemon (Citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. To investigate the...

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Autores principales: Hiramitsu, Masanori, Shimada, Yasuhito, Kuroyanagi, Junya, Inoue, Takashi, Katagiri, Takao, Zang, Liqing, Nishimura, Yuhei, Nishimura, Norihiro, Tanaka, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892443/
https://www.ncbi.nlm.nih.gov/pubmed/24424211
http://dx.doi.org/10.1038/srep03708
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author Hiramitsu, Masanori
Shimada, Yasuhito
Kuroyanagi, Junya
Inoue, Takashi
Katagiri, Takao
Zang, Liqing
Nishimura, Yuhei
Nishimura, Norihiro
Tanaka, Toshio
author_facet Hiramitsu, Masanori
Shimada, Yasuhito
Kuroyanagi, Junya
Inoue, Takashi
Katagiri, Takao
Zang, Liqing
Nishimura, Yuhei
Nishimura, Norihiro
Tanaka, Toshio
author_sort Hiramitsu, Masanori
collection PubMed
description Lemon (Citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. To investigate the mechanism of action of eriocitrin, we conducted feeding experiments on zebrafish with diet-induced obesity. Oral administration of eriocitrin (32 mg/kg/day for 28 days) improved dyslipidaemia and decreased lipid droplets in the liver. DNA microarray analysis revealed that eriocitrin increased mRNA of mitochondrial biogenesis genes, such as mitochondria transcription factor, nuclear respiratory factor 1, cytochrome c oxidase subunit 4, and ATP synthase. In HepG2 cells, eriocitrin also induced the corresponding orthologues, and reduced lipid accumulation under conditions of lipid loading. Eriocitrin increased mitochondrial size and mtDNA content, which resulted in ATP production in HepG2 cells and zebrafish. In summary, dietary eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis.
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spelling pubmed-38924432014-01-15 Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis Hiramitsu, Masanori Shimada, Yasuhito Kuroyanagi, Junya Inoue, Takashi Katagiri, Takao Zang, Liqing Nishimura, Yuhei Nishimura, Norihiro Tanaka, Toshio Sci Rep Article Lemon (Citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. To investigate the mechanism of action of eriocitrin, we conducted feeding experiments on zebrafish with diet-induced obesity. Oral administration of eriocitrin (32 mg/kg/day for 28 days) improved dyslipidaemia and decreased lipid droplets in the liver. DNA microarray analysis revealed that eriocitrin increased mRNA of mitochondrial biogenesis genes, such as mitochondria transcription factor, nuclear respiratory factor 1, cytochrome c oxidase subunit 4, and ATP synthase. In HepG2 cells, eriocitrin also induced the corresponding orthologues, and reduced lipid accumulation under conditions of lipid loading. Eriocitrin increased mitochondrial size and mtDNA content, which resulted in ATP production in HepG2 cells and zebrafish. In summary, dietary eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis. Nature Publishing Group 2014-01-15 /pmc/articles/PMC3892443/ /pubmed/24424211 http://dx.doi.org/10.1038/srep03708 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Hiramitsu, Masanori
Shimada, Yasuhito
Kuroyanagi, Junya
Inoue, Takashi
Katagiri, Takao
Zang, Liqing
Nishimura, Yuhei
Nishimura, Norihiro
Tanaka, Toshio
Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title_full Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title_fullStr Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title_full_unstemmed Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title_short Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
title_sort eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892443/
https://www.ncbi.nlm.nih.gov/pubmed/24424211
http://dx.doi.org/10.1038/srep03708
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