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Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer

An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30(®). Thiolated aptamer specific to...

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Autores principales: Evtugyn, Gennady, Porfireva, Anna, Stepanova, Veronika, Kutyreva, Marianna, Gataulina, Alfiya, Ulakhovich, Nikolay, Evtugyn, Vladimir, Hianik, Tibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892811/
https://www.ncbi.nlm.nih.gov/pubmed/24287535
http://dx.doi.org/10.3390/s131216129
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author Evtugyn, Gennady
Porfireva, Anna
Stepanova, Veronika
Kutyreva, Marianna
Gataulina, Alfiya
Ulakhovich, Nikolay
Evtugyn, Vladimir
Hianik, Tibor
author_facet Evtugyn, Gennady
Porfireva, Anna
Stepanova, Veronika
Kutyreva, Marianna
Gataulina, Alfiya
Ulakhovich, Nikolay
Evtugyn, Vladimir
Hianik, Tibor
author_sort Evtugyn, Gennady
collection PubMed
description An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30(®). Thiolated aptamer specific to OTA was covalently attached to Au nanoparticles via Au-S bonding. The interaction of the aptamer with OTA induced the conformational switch of the aptamer from linear to guanine quadruplex form followed by consolidation of the surface layer and an increase of the charge transfer resistance. The aptasensor makes it possible to detect from 0.1 to 100 nM of OTA (limit of detection: 0.02 nM) in the presence of at least 50 fold excess of ochratoxin B. The applicability of the aptasensor for real sample assay was confirmed by testing spiked beer samples. The recovery of 2 nM OTA was found to be 70% for light beer and 78% for dark beer.
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spelling pubmed-38928112014-01-16 Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer Evtugyn, Gennady Porfireva, Anna Stepanova, Veronika Kutyreva, Marianna Gataulina, Alfiya Ulakhovich, Nikolay Evtugyn, Vladimir Hianik, Tibor Sensors (Basel) Article An impedimetric aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with a new modifier consisting of electropolymerized Neutral Red and a mixture of Au nanoparticles suspended in the dendrimeric polymer Botlorn H30(®). Thiolated aptamer specific to OTA was covalently attached to Au nanoparticles via Au-S bonding. The interaction of the aptamer with OTA induced the conformational switch of the aptamer from linear to guanine quadruplex form followed by consolidation of the surface layer and an increase of the charge transfer resistance. The aptasensor makes it possible to detect from 0.1 to 100 nM of OTA (limit of detection: 0.02 nM) in the presence of at least 50 fold excess of ochratoxin B. The applicability of the aptasensor for real sample assay was confirmed by testing spiked beer samples. The recovery of 2 nM OTA was found to be 70% for light beer and 78% for dark beer. Molecular Diversity Preservation International (MDPI) 2013-11-26 /pmc/articles/PMC3892811/ /pubmed/24287535 http://dx.doi.org/10.3390/s131216129 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Evtugyn, Gennady
Porfireva, Anna
Stepanova, Veronika
Kutyreva, Marianna
Gataulina, Alfiya
Ulakhovich, Nikolay
Evtugyn, Vladimir
Hianik, Tibor
Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title_full Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title_fullStr Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title_full_unstemmed Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title_short Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
title_sort impedimetric aptasensor for ochratoxin a determination based on au nanoparticles stabilized with hyper-branched polymer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3892811/
https://www.ncbi.nlm.nih.gov/pubmed/24287535
http://dx.doi.org/10.3390/s131216129
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