An Efficient Large-Scale Retroviral Transduction Method Involving Preloading the Vector into a RetroNectin-Coated Bag with Low-Temperature Shaking

In retroviral vector-mediated gene transfer, transduction efficiency can be hampered by inhibitory molecules derived from the culture fluid of virus producer cell lines. To remove these inhibitory molecules to enable better gene transduction, we had previously developed a transduction method using a...

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Detalles Bibliográficos
Autores principales: Dodo, Katsuyuki, Chono, Hideto, Saito, Naoki, Tanaka, Yoshinori, Tahara, Kenichi, Nukaya, Ikuei, Mineno, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893289/
https://www.ncbi.nlm.nih.gov/pubmed/24454964
http://dx.doi.org/10.1371/journal.pone.0086275

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893289/
https://www.ncbi.nlm.nih.gov/pubmed/24454964
http://dx.doi.org/10.1371/journal.pone.0086275

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