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Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments
We tested for chemical reagents that would be useful in preparing a large number of vital single cells from colonial Botryococcus braunii B-race, variety Showa. Among the 18 reagents assayed, glycerol and erythritol showed the highest potency for releasing single cells. Incubation in medium containi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893471/ https://www.ncbi.nlm.nih.gov/pubmed/23943006 http://dx.doi.org/10.1007/s00709-013-0537-4 |
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author | Hou, Liyuan Park, Hyunsun Okada, Shigeru Ohama, Takeshi |
author_facet | Hou, Liyuan Park, Hyunsun Okada, Shigeru Ohama, Takeshi |
author_sort | Hou, Liyuan |
collection | PubMed |
description | We tested for chemical reagents that would be useful in preparing a large number of vital single cells from colonial Botryococcus braunii B-race, variety Showa. Among the 18 reagents assayed, glycerol and erythritol showed the highest potency for releasing single cells. Incubation in medium containing these reagents released 40–50 % single cells in 15 min. Fluorescent staining with Nile red revealed that except for the cap-like structures the released single cells were free of hydrocarbon oils that accumulated in the extracellular matrix where the single cells were embedded. However, to maintain the prepared single cells in vital condition, they must be maintained at a high concentration (>2 × 10(7) cells/ml); at low concentrations, they rapidly lost chlorophyll and get disrupted. In contrast to the above results obtained using B-race, Showa, single cells prepared from A-race varieties survived even at low cell concentrations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00709-013-0537-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3893471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-38934712014-01-22 Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments Hou, Liyuan Park, Hyunsun Okada, Shigeru Ohama, Takeshi Protoplasma Original Article We tested for chemical reagents that would be useful in preparing a large number of vital single cells from colonial Botryococcus braunii B-race, variety Showa. Among the 18 reagents assayed, glycerol and erythritol showed the highest potency for releasing single cells. Incubation in medium containing these reagents released 40–50 % single cells in 15 min. Fluorescent staining with Nile red revealed that except for the cap-like structures the released single cells were free of hydrocarbon oils that accumulated in the extracellular matrix where the single cells were embedded. However, to maintain the prepared single cells in vital condition, they must be maintained at a high concentration (>2 × 10(7) cells/ml); at low concentrations, they rapidly lost chlorophyll and get disrupted. In contrast to the above results obtained using B-race, Showa, single cells prepared from A-race varieties survived even at low cell concentrations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00709-013-0537-4) contains supplementary material, which is available to authorized users. Springer Vienna 2013-08-13 2014 /pmc/articles/PMC3893471/ /pubmed/23943006 http://dx.doi.org/10.1007/s00709-013-0537-4 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Article Hou, Liyuan Park, Hyunsun Okada, Shigeru Ohama, Takeshi Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title | Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title_full | Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title_fullStr | Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title_full_unstemmed | Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title_short | Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments |
title_sort | release of single cells from the colonial oil-producing alga botryococcus braunii by chemical treatments |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893471/ https://www.ncbi.nlm.nih.gov/pubmed/23943006 http://dx.doi.org/10.1007/s00709-013-0537-4 |
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