Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

BACKGROUND: An industrial approach to protein production demands maximization of cloned gene expression, balanced with the recombinant host’s viability. Expression of toxic genes from thermophiles poses particular difficulties due to high GC content, mRNA secondary structures, rare codon usage and i...

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Detalles Bibliográficos
Autores principales: Zylicz-Stachula, Agnieszka, Zolnierkiewicz, Olga, Sliwinska, Katarzyna, Jezewska-Frackowiak, Joanna, Skowron, Piotr M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893498/
https://www.ncbi.nlm.nih.gov/pubmed/24410856
http://dx.doi.org/10.1186/1475-2859-13-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893498/
https://www.ncbi.nlm.nih.gov/pubmed/24410856
http://dx.doi.org/10.1186/1475-2859-13-7

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