Cargando…

Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer

BACKGROUND: Besides gene duplication and de novo gene generation, horizontal gene transfer (HGT) is another important way of acquiring new genes. HGT may endow the recipients with novel phenotypic traits that are important for species evolution and adaption to new ecological niches. Parasitic system...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Dale, Qi, Jinfeng, Yue, Jipei, Huang, Jinling, Sun, Ting, Li, Suoping, Wen, Jian-Fan, Hettenhausen, Christian, Wu, Jinsong, Wang, Lei, Zhuang, Huifu, Wu, Jianqiang, Sun, Guiling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893544/
https://www.ncbi.nlm.nih.gov/pubmed/24411025
http://dx.doi.org/10.1186/1471-2229-14-19
_version_ 1782299712131432448
author Zhang, Dale
Qi, Jinfeng
Yue, Jipei
Huang, Jinling
Sun, Ting
Li, Suoping
Wen, Jian-Fan
Hettenhausen, Christian
Wu, Jinsong
Wang, Lei
Zhuang, Huifu
Wu, Jianqiang
Sun, Guiling
author_facet Zhang, Dale
Qi, Jinfeng
Yue, Jipei
Huang, Jinling
Sun, Ting
Li, Suoping
Wen, Jian-Fan
Hettenhausen, Christian
Wu, Jinsong
Wang, Lei
Zhuang, Huifu
Wu, Jianqiang
Sun, Guiling
author_sort Zhang, Dale
collection PubMed
description BACKGROUND: Besides gene duplication and de novo gene generation, horizontal gene transfer (HGT) is another important way of acquiring new genes. HGT may endow the recipients with novel phenotypic traits that are important for species evolution and adaption to new ecological niches. Parasitic systems expectedly allow the occurrence of HGT at relatively high frequencies due to their long-term physical contact. In plants, a number of HGT events have been reported between the organelles of parasites and the hosts, but HGT between host and parasite nuclear genomes has rarely been found. RESULTS: A thorough transcriptome screening revealed that a strictosidine synthase-like (SSL) gene in the root parasitic plant Orobanche aegyptiaca and the shoot parasitic plant Cuscuta australis showed much higher sequence similarities with those in Brassicaceae than with those in their close relatives, suggesting independent gene horizontal transfer events from Brassicaceae to these parasites. These findings were strongly supported by phylogenetic analysis and their identical unique amino acid residues and deletions. Intriguingly, the nucleus-located SSL genes in Brassicaceae belonged to a new member of SSL gene family, which were originated from gene duplication. The presence of introns indicated that the transfer occurred directly by DNA integration in both parasites. Furthermore, positive selection was detected in the foreign SSL gene in O. aegyptiaca but not in C. australis. The expression of the foreign SSL genes in these two parasitic plants was detected in multiple development stages and tissues, and the foreign SSL gene was induced after wounding treatment in C. australis stems. These data imply that the foreign genes may still retain certain functions in the recipient species. CONCLUSIONS: Our study strongly supports that parasitic plants can gain novel nuclear genes from distantly related host species by HGT and the foreign genes may execute certain functions in the new hosts.
format Online
Article
Text
id pubmed-3893544
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38935442014-01-17 Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer Zhang, Dale Qi, Jinfeng Yue, Jipei Huang, Jinling Sun, Ting Li, Suoping Wen, Jian-Fan Hettenhausen, Christian Wu, Jinsong Wang, Lei Zhuang, Huifu Wu, Jianqiang Sun, Guiling BMC Plant Biol Research Article BACKGROUND: Besides gene duplication and de novo gene generation, horizontal gene transfer (HGT) is another important way of acquiring new genes. HGT may endow the recipients with novel phenotypic traits that are important for species evolution and adaption to new ecological niches. Parasitic systems expectedly allow the occurrence of HGT at relatively high frequencies due to their long-term physical contact. In plants, a number of HGT events have been reported between the organelles of parasites and the hosts, but HGT between host and parasite nuclear genomes has rarely been found. RESULTS: A thorough transcriptome screening revealed that a strictosidine synthase-like (SSL) gene in the root parasitic plant Orobanche aegyptiaca and the shoot parasitic plant Cuscuta australis showed much higher sequence similarities with those in Brassicaceae than with those in their close relatives, suggesting independent gene horizontal transfer events from Brassicaceae to these parasites. These findings were strongly supported by phylogenetic analysis and their identical unique amino acid residues and deletions. Intriguingly, the nucleus-located SSL genes in Brassicaceae belonged to a new member of SSL gene family, which were originated from gene duplication. The presence of introns indicated that the transfer occurred directly by DNA integration in both parasites. Furthermore, positive selection was detected in the foreign SSL gene in O. aegyptiaca but not in C. australis. The expression of the foreign SSL genes in these two parasitic plants was detected in multiple development stages and tissues, and the foreign SSL gene was induced after wounding treatment in C. australis stems. These data imply that the foreign genes may still retain certain functions in the recipient species. CONCLUSIONS: Our study strongly supports that parasitic plants can gain novel nuclear genes from distantly related host species by HGT and the foreign genes may execute certain functions in the new hosts. BioMed Central 2014-01-13 /pmc/articles/PMC3893544/ /pubmed/24411025 http://dx.doi.org/10.1186/1471-2229-14-19 Text en Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Dale
Qi, Jinfeng
Yue, Jipei
Huang, Jinling
Sun, Ting
Li, Suoping
Wen, Jian-Fan
Hettenhausen, Christian
Wu, Jinsong
Wang, Lei
Zhuang, Huifu
Wu, Jianqiang
Sun, Guiling
Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title_full Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title_fullStr Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title_full_unstemmed Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title_short Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
title_sort root parasitic plant orobanche aegyptiaca and shoot parasitic plant cuscuta australis obtained brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893544/
https://www.ncbi.nlm.nih.gov/pubmed/24411025
http://dx.doi.org/10.1186/1471-2229-14-19
work_keys_str_mv AT zhangdale rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT qijinfeng rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT yuejipei rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT huangjinling rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT sunting rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT lisuoping rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT wenjianfan rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT hettenhausenchristian rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT wujinsong rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT wanglei rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT zhuanghuifu rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT wujianqiang rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer
AT sunguiling rootparasiticplantorobancheaegyptiacaandshootparasiticplantcuscutaaustralisobtainedbrassicaceaespecificstrictosidinesynthaselikegenesbyhorizontalgenetransfer