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Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit

The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in...

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Autores principales: da Silva, Manassés Daniel, Silva, Roberta Lane de Oliveira, Costa Ferreira Neto, José Ribamar, Guimarães, Ana Carolina Ribeiro, Veiga, Daniela Truffi, Chabregas, Sabrina Moutinho, Burnquist, William Lee, Kahl, Günter, Benko-Iseppon, Ana Maria, Kido, Ederson Akio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893750/
https://www.ncbi.nlm.nih.gov/pubmed/24490055
http://dx.doi.org/10.1155/2013/763945
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author da Silva, Manassés Daniel
Silva, Roberta Lane de Oliveira
Costa Ferreira Neto, José Ribamar
Guimarães, Ana Carolina Ribeiro
Veiga, Daniela Truffi
Chabregas, Sabrina Moutinho
Burnquist, William Lee
Kahl, Günter
Benko-Iseppon, Ana Maria
Kido, Ederson Akio
author_facet da Silva, Manassés Daniel
Silva, Roberta Lane de Oliveira
Costa Ferreira Neto, José Ribamar
Guimarães, Ana Carolina Ribeiro
Veiga, Daniela Truffi
Chabregas, Sabrina Moutinho
Burnquist, William Lee
Kahl, Günter
Benko-Iseppon, Ana Maria
Kido, Ederson Akio
author_sort da Silva, Manassés Daniel
collection PubMed
description The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.
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spelling pubmed-38937502014-02-02 Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit da Silva, Manassés Daniel Silva, Roberta Lane de Oliveira Costa Ferreira Neto, José Ribamar Guimarães, Ana Carolina Ribeiro Veiga, Daniela Truffi Chabregas, Sabrina Moutinho Burnquist, William Lee Kahl, Günter Benko-Iseppon, Ana Maria Kido, Ederson Akio J Nucleic Acids Research Article The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific. Hindawi Publishing Corporation 2013 2013-12-29 /pmc/articles/PMC3893750/ /pubmed/24490055 http://dx.doi.org/10.1155/2013/763945 Text en Copyright © 2013 Manassés Daniel da Silva et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
da Silva, Manassés Daniel
Silva, Roberta Lane de Oliveira
Costa Ferreira Neto, José Ribamar
Guimarães, Ana Carolina Ribeiro
Veiga, Daniela Truffi
Chabregas, Sabrina Moutinho
Burnquist, William Lee
Kahl, Günter
Benko-Iseppon, Ana Maria
Kido, Ederson Akio
Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title_full Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title_fullStr Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title_full_unstemmed Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title_short Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
title_sort expression analysis of sugarcane aquaporin genes under water deficit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893750/
https://www.ncbi.nlm.nih.gov/pubmed/24490055
http://dx.doi.org/10.1155/2013/763945
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