Cargando…
Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioa...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893781/ https://www.ncbi.nlm.nih.gov/pubmed/24489585 http://dx.doi.org/10.1155/2013/560417 |
_version_ | 1782299749911625728 |
---|---|
author | Kim, Jong-Woo Park, Sang Jin Lim, Jong Hwan Yang, Jae Won Shin, Jung Cheul Lee, Sang Wook Suh, Joo Won Hwang, Soon B. |
author_facet | Kim, Jong-Woo Park, Sang Jin Lim, Jong Hwan Yang, Jae Won Shin, Jung Cheul Lee, Sang Wook Suh, Joo Won Hwang, Soon B. |
author_sort | Kim, Jong-Woo |
collection | PubMed |
description | Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioactive compounds against HCV. The aim of this study was to evaluate the functional effects of triterpenoid saponins contained in the root extract of Platycodon grandiflorum (PG) on viral enzyme activities and replication in both HCV replicon cells and cell culture grown HCV- (HCVcc-) infected cells. Inhibitory activities of triterpenoid saponins from PG were verified by NS5B RNA-dependent RNA polymerase assay and were further confirmed in the context of HCV replication. Six triterpenoid saponins (platycodin D, platycodin D(2), platycodin D(3), deapioplatycodin D, deapioplatycodin D(2), and platyconic acid A), PG saponin mixture (PGSM), were identified as active components exerting anti-HCV activity. Importantly, PGSM exerted synergistic anti-HCV activity in combination with either interferon-α or NS5A inhibitors. We demonstrated that combinatorial treatment of PGSM and IFN-α efficiently suppressed colony formation with significant reduction in drug resistant variant of HCV. These data suggest that triterpenoid saponin may represent a novel anti-HCV therapeutic agent. |
format | Online Article Text |
id | pubmed-3893781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38937812014-02-02 Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication Kim, Jong-Woo Park, Sang Jin Lim, Jong Hwan Yang, Jae Won Shin, Jung Cheul Lee, Sang Wook Suh, Joo Won Hwang, Soon B. Evid Based Complement Alternat Med Research Article Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioactive compounds against HCV. The aim of this study was to evaluate the functional effects of triterpenoid saponins contained in the root extract of Platycodon grandiflorum (PG) on viral enzyme activities and replication in both HCV replicon cells and cell culture grown HCV- (HCVcc-) infected cells. Inhibitory activities of triterpenoid saponins from PG were verified by NS5B RNA-dependent RNA polymerase assay and were further confirmed in the context of HCV replication. Six triterpenoid saponins (platycodin D, platycodin D(2), platycodin D(3), deapioplatycodin D, deapioplatycodin D(2), and platyconic acid A), PG saponin mixture (PGSM), were identified as active components exerting anti-HCV activity. Importantly, PGSM exerted synergistic anti-HCV activity in combination with either interferon-α or NS5A inhibitors. We demonstrated that combinatorial treatment of PGSM and IFN-α efficiently suppressed colony formation with significant reduction in drug resistant variant of HCV. These data suggest that triterpenoid saponin may represent a novel anti-HCV therapeutic agent. Hindawi Publishing Corporation 2013 2013-12-15 /pmc/articles/PMC3893781/ /pubmed/24489585 http://dx.doi.org/10.1155/2013/560417 Text en Copyright © 2013 Jong-Woo Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kim, Jong-Woo Park, Sang Jin Lim, Jong Hwan Yang, Jae Won Shin, Jung Cheul Lee, Sang Wook Suh, Joo Won Hwang, Soon B. Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title | Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title_full | Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title_fullStr | Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title_full_unstemmed | Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title_short | Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication |
title_sort | triterpenoid saponins isolated from platycodon grandiflorum inhibit hepatitis c virus replication |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893781/ https://www.ncbi.nlm.nih.gov/pubmed/24489585 http://dx.doi.org/10.1155/2013/560417 |
work_keys_str_mv | AT kimjongwoo triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT parksangjin triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT limjonghwan triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT yangjaewon triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT shinjungcheul triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT leesangwook triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT suhjoowon triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication AT hwangsoonb triterpenoidsaponinsisolatedfromplatycodongrandifloruminhibithepatitiscvirusreplication |