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Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication

Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioa...

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Autores principales: Kim, Jong-Woo, Park, Sang Jin, Lim, Jong Hwan, Yang, Jae Won, Shin, Jung Cheul, Lee, Sang Wook, Suh, Joo Won, Hwang, Soon B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893781/
https://www.ncbi.nlm.nih.gov/pubmed/24489585
http://dx.doi.org/10.1155/2013/560417
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author Kim, Jong-Woo
Park, Sang Jin
Lim, Jong Hwan
Yang, Jae Won
Shin, Jung Cheul
Lee, Sang Wook
Suh, Joo Won
Hwang, Soon B.
author_facet Kim, Jong-Woo
Park, Sang Jin
Lim, Jong Hwan
Yang, Jae Won
Shin, Jung Cheul
Lee, Sang Wook
Suh, Joo Won
Hwang, Soon B.
author_sort Kim, Jong-Woo
collection PubMed
description Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioactive compounds against HCV. The aim of this study was to evaluate the functional effects of triterpenoid saponins contained in the root extract of Platycodon grandiflorum (PG) on viral enzyme activities and replication in both HCV replicon cells and cell culture grown HCV- (HCVcc-) infected cells. Inhibitory activities of triterpenoid saponins from PG were verified by NS5B RNA-dependent RNA polymerase assay and were further confirmed in the context of HCV replication. Six triterpenoid saponins (platycodin D, platycodin D(2), platycodin D(3), deapioplatycodin D, deapioplatycodin D(2), and platyconic acid A), PG saponin mixture (PGSM), were identified as active components exerting anti-HCV activity. Importantly, PGSM exerted synergistic anti-HCV activity in combination with either interferon-α or NS5A inhibitors. We demonstrated that combinatorial treatment of PGSM and IFN-α efficiently suppressed colony formation with significant reduction in drug resistant variant of HCV. These data suggest that triterpenoid saponin may represent a novel anti-HCV therapeutic agent.
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spelling pubmed-38937812014-02-02 Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication Kim, Jong-Woo Park, Sang Jin Lim, Jong Hwan Yang, Jae Won Shin, Jung Cheul Lee, Sang Wook Suh, Joo Won Hwang, Soon B. Evid Based Complement Alternat Med Research Article Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioactive compounds against HCV. The aim of this study was to evaluate the functional effects of triterpenoid saponins contained in the root extract of Platycodon grandiflorum (PG) on viral enzyme activities and replication in both HCV replicon cells and cell culture grown HCV- (HCVcc-) infected cells. Inhibitory activities of triterpenoid saponins from PG were verified by NS5B RNA-dependent RNA polymerase assay and were further confirmed in the context of HCV replication. Six triterpenoid saponins (platycodin D, platycodin D(2), platycodin D(3), deapioplatycodin D, deapioplatycodin D(2), and platyconic acid A), PG saponin mixture (PGSM), were identified as active components exerting anti-HCV activity. Importantly, PGSM exerted synergistic anti-HCV activity in combination with either interferon-α or NS5A inhibitors. We demonstrated that combinatorial treatment of PGSM and IFN-α efficiently suppressed colony formation with significant reduction in drug resistant variant of HCV. These data suggest that triterpenoid saponin may represent a novel anti-HCV therapeutic agent. Hindawi Publishing Corporation 2013 2013-12-15 /pmc/articles/PMC3893781/ /pubmed/24489585 http://dx.doi.org/10.1155/2013/560417 Text en Copyright © 2013 Jong-Woo Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Jong-Woo
Park, Sang Jin
Lim, Jong Hwan
Yang, Jae Won
Shin, Jung Cheul
Lee, Sang Wook
Suh, Joo Won
Hwang, Soon B.
Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title_full Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title_fullStr Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title_full_unstemmed Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title_short Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
title_sort triterpenoid saponins isolated from platycodon grandiflorum inhibit hepatitis c virus replication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893781/
https://www.ncbi.nlm.nih.gov/pubmed/24489585
http://dx.doi.org/10.1155/2013/560417
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