Cargando…
Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System
Nonviral gene delivery systems are rapidly becoming a desirable and applicable method to overexpress genes in various types of cells. We have recently developed a piggyBac transposase-based, helper-independent and self-inactivating delivery system (pmGENIE-3) capable of high-efficiency transfection...
Autores principales: | Owens, Jesse B, Mathews, Juanita, Davy, Philip, Stoytchev, Ilko, Moisyadi, Stefan, Allsopp, Rich |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894583/ https://www.ncbi.nlm.nih.gov/pubmed/24326734 http://dx.doi.org/10.1038/mtna.2013.61 |
Ejemplares similares
-
Chimeric piggyBac transposases for genomic targeting in human cells
por: Owens, Jesse B., et al.
Publicado: (2012) -
RNA-guided piggyBac transposition in human cells
por: Hew, Brian E, et al.
Publicado: (2019) -
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells
por: Owens, Jesse B., et al.
Publicado: (2013) -
Transpositional transgenesis with piggyBac
por: Urschitz, Johann, et al.
Publicado: (2013) -
Structural basis of seamless excision and specific targeting by piggyBac transposase
por: Chen, Qiujia, et al.
Publicado: (2020)