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Investigation of the actin scavenging system in pre-eclampsia()

OBJECTIVES: Cell injury releases actin, the most abundant cell protein. Gelsolin and vitamin D binding protein (VDBP) together depolymerise and clear cell-free actin. Impaired actin clearance is associated with several diseases and correlates with clinical outcome. The actin scavenging system was in...

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Autores principales: Tannetta, Dionne S., Redman, Christopher W., Sargent, Ian L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Scientific Publishers 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894683/
https://www.ncbi.nlm.nih.gov/pubmed/24239294
http://dx.doi.org/10.1016/j.ejogrb.2013.10.022
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author Tannetta, Dionne S.
Redman, Christopher W.
Sargent, Ian L.
author_facet Tannetta, Dionne S.
Redman, Christopher W.
Sargent, Ian L.
author_sort Tannetta, Dionne S.
collection PubMed
description OBJECTIVES: Cell injury releases actin, the most abundant cell protein. Gelsolin and vitamin D binding protein (VDBP) together depolymerise and clear cell-free actin. Impaired actin clearance is associated with several diseases and correlates with clinical outcome. The actin scavenging system was investigated in pre-eclampsia (PE), a procoagulant and proinflammatory state with placental and vascular damage. STUDY DESIGN: Plasma gelsolin and actin free VDBP (AFVDBP) were measured in PE (early onset <33 weeks; late onset ≥36 weeks), matched normal pregnant (normP) and non-pregnant (nonPr) women, using commercially available ELISAs. Longitudinal samples from normP and women who subsequently developed PE were also analysed. RESULTS: Plasma gelsolin fell during pregnancy (p = 0.0002), with a concomitant rise in actin-free VDBP (p < 0.001). Gelsolin concentrations were only significantly lower in established PE (p < 0.05) when compared to non-pregnant controls. CONCLUSIONS: We have shown that the components of the actin clearance system, gelsolin and AFVDBP, are altered in normal pregnancy and further changes occur in established PE, suggesting depleted actin clearance in PE. Whether this is a cause or consequence of PE pathophysiology requires further investigation.
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spelling pubmed-38946832014-01-17 Investigation of the actin scavenging system in pre-eclampsia() Tannetta, Dionne S. Redman, Christopher W. Sargent, Ian L. Eur J Obstet Gynecol Reprod Biol Article OBJECTIVES: Cell injury releases actin, the most abundant cell protein. Gelsolin and vitamin D binding protein (VDBP) together depolymerise and clear cell-free actin. Impaired actin clearance is associated with several diseases and correlates with clinical outcome. The actin scavenging system was investigated in pre-eclampsia (PE), a procoagulant and proinflammatory state with placental and vascular damage. STUDY DESIGN: Plasma gelsolin and actin free VDBP (AFVDBP) were measured in PE (early onset <33 weeks; late onset ≥36 weeks), matched normal pregnant (normP) and non-pregnant (nonPr) women, using commercially available ELISAs. Longitudinal samples from normP and women who subsequently developed PE were also analysed. RESULTS: Plasma gelsolin fell during pregnancy (p = 0.0002), with a concomitant rise in actin-free VDBP (p < 0.001). Gelsolin concentrations were only significantly lower in established PE (p < 0.05) when compared to non-pregnant controls. CONCLUSIONS: We have shown that the components of the actin clearance system, gelsolin and AFVDBP, are altered in normal pregnancy and further changes occur in established PE, suggesting depleted actin clearance in PE. Whether this is a cause or consequence of PE pathophysiology requires further investigation. Elsevier Scientific Publishers 2014-01 /pmc/articles/PMC3894683/ /pubmed/24239294 http://dx.doi.org/10.1016/j.ejogrb.2013.10.022 Text en © 2013 The Authors https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open access article under the CC BY NC ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Tannetta, Dionne S.
Redman, Christopher W.
Sargent, Ian L.
Investigation of the actin scavenging system in pre-eclampsia()
title Investigation of the actin scavenging system in pre-eclampsia()
title_full Investigation of the actin scavenging system in pre-eclampsia()
title_fullStr Investigation of the actin scavenging system in pre-eclampsia()
title_full_unstemmed Investigation of the actin scavenging system in pre-eclampsia()
title_short Investigation of the actin scavenging system in pre-eclampsia()
title_sort investigation of the actin scavenging system in pre-eclampsia()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894683/
https://www.ncbi.nlm.nih.gov/pubmed/24239294
http://dx.doi.org/10.1016/j.ejogrb.2013.10.022
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