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High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo

Precise spatial and temporal manipulation of neural activity in specific genetically defined cell populations is now possible with the advent of optogenetics. The emerging field of optogenetics consists of a set of naturally-occurring and engineered light-sensitive membrane proteins that are able to...

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Autores principales: Nakamura, Shinya, Baratta, Michael V, Pomrenze, Matthew B, Dolzani, Samuel D, Cooper, Donald C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894804/
https://www.ncbi.nlm.nih.gov/pubmed/24555016
http://dx.doi.org/10.12688/f1000research.1-7.v1
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author Nakamura, Shinya
Baratta, Michael V
Pomrenze, Matthew B
Dolzani, Samuel D
Cooper, Donald C
author_facet Nakamura, Shinya
Baratta, Michael V
Pomrenze, Matthew B
Dolzani, Samuel D
Cooper, Donald C
author_sort Nakamura, Shinya
collection PubMed
description Precise spatial and temporal manipulation of neural activity in specific genetically defined cell populations is now possible with the advent of optogenetics. The emerging field of optogenetics consists of a set of naturally-occurring and engineered light-sensitive membrane proteins that are able to activate (e.g. channelrhodopsin-2, ChR2) or silence (e.g. halorhodopsin, NpHR) neural activity. Here we demonstrate the technique and the feasibility of using novel adeno-associated viral (AAV) tools to activate (AAV-CaMKllα-ChR2-eYFP) or silence (AAV-CaMKllα-eNpHR3.0-eYFP) neural activity of rat prefrontal cortical prelimbic (PL) pyramidal neurons  in vivo.  In vivo single unit extracellular recording of ChR2-transduced pyramidal neurons showed that delivery of brief (10 ms) blue (473 nm) light-pulse trains up to 20 Hz via a custom fiber optic-coupled recording electrode (optrode) induced spiking with high fidelity at 20 Hz for the duration of recording (up to two hours in some cases). To silence spontaneously active neurons, we transduced them with the NpHR construct and administered continuous green (532 nm) light to completely inhibit action potential activity for up to 10 seconds with 100% fidelity in most cases. These versatile photosensitive tools, combined with optrode recording methods, provide experimental control over activity of genetically defined neurons and can be used to investigate the functional relationship between neural activity and complex cognitive behavior.
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spelling pubmed-38948042014-01-28 High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo Nakamura, Shinya Baratta, Michael V Pomrenze, Matthew B Dolzani, Samuel D Cooper, Donald C F1000Res Research Article Precise spatial and temporal manipulation of neural activity in specific genetically defined cell populations is now possible with the advent of optogenetics. The emerging field of optogenetics consists of a set of naturally-occurring and engineered light-sensitive membrane proteins that are able to activate (e.g. channelrhodopsin-2, ChR2) or silence (e.g. halorhodopsin, NpHR) neural activity. Here we demonstrate the technique and the feasibility of using novel adeno-associated viral (AAV) tools to activate (AAV-CaMKllα-ChR2-eYFP) or silence (AAV-CaMKllα-eNpHR3.0-eYFP) neural activity of rat prefrontal cortical prelimbic (PL) pyramidal neurons  in vivo.  In vivo single unit extracellular recording of ChR2-transduced pyramidal neurons showed that delivery of brief (10 ms) blue (473 nm) light-pulse trains up to 20 Hz via a custom fiber optic-coupled recording electrode (optrode) induced spiking with high fidelity at 20 Hz for the duration of recording (up to two hours in some cases). To silence spontaneously active neurons, we transduced them with the NpHR construct and administered continuous green (532 nm) light to completely inhibit action potential activity for up to 10 seconds with 100% fidelity in most cases. These versatile photosensitive tools, combined with optrode recording methods, provide experimental control over activity of genetically defined neurons and can be used to investigate the functional relationship between neural activity and complex cognitive behavior. F1000Research 2012-07-30 /pmc/articles/PMC3894804/ /pubmed/24555016 http://dx.doi.org/10.12688/f1000research.1-7.v1 Text en Copyright: © 2012 Nakamura S et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/publicdomain/zero/1.0/Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
spellingShingle Research Article
Nakamura, Shinya
Baratta, Michael V
Pomrenze, Matthew B
Dolzani, Samuel D
Cooper, Donald C
High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title_full High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title_fullStr High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title_full_unstemmed High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title_short High fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
title_sort high fidelity optogenetic control of individual prefrontal cortical pyramidal neurons in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3894804/
https://www.ncbi.nlm.nih.gov/pubmed/24555016
http://dx.doi.org/10.12688/f1000research.1-7.v1
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