EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7

BACKGROUND AND OBJECTIVE: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (HUS). Cattle are an important reservoir of EHEC. This research aims at vaccination with a...

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Autores principales: Rad, Hamid Sedighian, Mousavi, Seyed Latif, Rasooli, Iraj, Amani, Jafar, Nadooshan, Moohamad Reza Jalali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895562/
https://www.ncbi.nlm.nih.gov/pubmed/24475331
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author Rad, Hamid Sedighian
Mousavi, Seyed Latif
Rasooli, Iraj
Amani, Jafar
Nadooshan, Moohamad Reza Jalali
author_facet Rad, Hamid Sedighian
Mousavi, Seyed Latif
Rasooli, Iraj
Amani, Jafar
Nadooshan, Moohamad Reza Jalali
author_sort Rad, Hamid Sedighian
collection PubMed
description BACKGROUND AND OBJECTIVE: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (HUS). Cattle are an important reservoir of EHEC. This research aims at vaccination with a divalent chimer protein composed of EspA(120) and Intimin 282 and its preventive effect of EHEC O157 colonization in mice rectal epithelium. MATERIALS AND METHODS: A divalent recombinant EspA-Intimin (EI) protein containing EspA(120) and Intimin(280) attached with a linker was amplified from a trivalent construct and cloned in pET-28a (+) vector. The immunization was conducted in mice after expression and purification of the recombinant EI (rEI). RESULTS: Mice subcutaneously immunized with rEI, elicited significant rEI specific serum IgG antibodies and showed significantly decreased E.coli O157:H7 shedding compared to the control group. CONCLUSION: The chimeric recombinant protein induced strong humoral response as well as protection against oral challenges with live E.coli O157:H7.
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spelling pubmed-38955622014-01-28 EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7 Rad, Hamid Sedighian Mousavi, Seyed Latif Rasooli, Iraj Amani, Jafar Nadooshan, Moohamad Reza Jalali Iran J Microbiol Original Article BACKGROUND AND OBJECTIVE: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (HUS). Cattle are an important reservoir of EHEC. This research aims at vaccination with a divalent chimer protein composed of EspA(120) and Intimin 282 and its preventive effect of EHEC O157 colonization in mice rectal epithelium. MATERIALS AND METHODS: A divalent recombinant EspA-Intimin (EI) protein containing EspA(120) and Intimin(280) attached with a linker was amplified from a trivalent construct and cloned in pET-28a (+) vector. The immunization was conducted in mice after expression and purification of the recombinant EI (rEI). RESULTS: Mice subcutaneously immunized with rEI, elicited significant rEI specific serum IgG antibodies and showed significantly decreased E.coli O157:H7 shedding compared to the control group. CONCLUSION: The chimeric recombinant protein induced strong humoral response as well as protection against oral challenges with live E.coli O157:H7. Tehran University of Medical Sciences 2013-09 /pmc/articles/PMC3895562/ /pubmed/24475331 Text en © 2013 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rad, Hamid Sedighian
Mousavi, Seyed Latif
Rasooli, Iraj
Amani, Jafar
Nadooshan, Moohamad Reza Jalali
EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title_full EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title_fullStr EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title_full_unstemmed EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title_short EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7
title_sort espa-intimin chimeric protein, a candidate vaccine against escherichia coli o157:h7
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895562/
https://www.ncbi.nlm.nih.gov/pubmed/24475331
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