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KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway

As a metastasis suppressor, KiSS1 has been implicated in numerous human cancers. However, recent studies have demonstrated that KiSS1 promotes tumor growth and metastasis in breast cancer, and it is unclear about the expression and function of KiSS1 in human osteosarcoma (OS). The aim of the present...

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Autores principales: Zhang, Y., Tang, Y.J., Li, Z.H., Pan, F., Huang, K., Xu, G.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896032/
https://www.ncbi.nlm.nih.gov/pubmed/24441183
http://dx.doi.org/10.4081/ejh.2013.e30
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author Zhang, Y.
Tang, Y.J.
Li, Z.H.
Pan, F.
Huang, K.
Xu, G.H.
author_facet Zhang, Y.
Tang, Y.J.
Li, Z.H.
Pan, F.
Huang, K.
Xu, G.H.
author_sort Zhang, Y.
collection PubMed
description As a metastasis suppressor, KiSS1 has been implicated in numerous human cancers. However, recent studies have demonstrated that KiSS1 promotes tumor growth and metastasis in breast cancer, and it is unclear about the expression and function of KiSS1 in human osteosarcoma (OS). The aim of the present study was to investigate the role and molecular mechanisms of KiSS1 in human OS. The expression of KiSS1 was assessed by immunohistochemical assay using a tissue microarray procedure in forty cases of OS tissues. A gain-of-function approach was used to observe the effects of lentiviral vector-mediated overexpression of KiSS1 (Lv-KiSS1) on the biological behaviors including proliferative activities and invasive potential of OS MG-63 cells, indicated by MTT and Transwell assays, respectively. The results showed that the expression of KiSS1 protein in OS tissues was significantly lowered compared to that in adjacent non-cancerous tissues (42.5% vs 70.0%, P=0.023), and had negative correlation with distant metastases of the tumor (P=0.019). Overexpression of KiSS1 inhibited proliferation and invasion of OS cells with the decreased expression of p38 MAPK and matrix metalloproteinase-9 (MMP-9). Taken together, our findings indicate that the decreased expression of KiSS1 is correlated with distant metastasis of OS, and KiSS1 may function as a tumor suppressor in OS cells through inhibition of the MAPK pathway, suggesting that KiSS1 may serve as a potential therapeutic target for the treatment of cancer.
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spelling pubmed-38960322014-01-24 KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway Zhang, Y. Tang, Y.J. Li, Z.H. Pan, F. Huang, K. Xu, G.H. Eur J Histochem Original Paper As a metastasis suppressor, KiSS1 has been implicated in numerous human cancers. However, recent studies have demonstrated that KiSS1 promotes tumor growth and metastasis in breast cancer, and it is unclear about the expression and function of KiSS1 in human osteosarcoma (OS). The aim of the present study was to investigate the role and molecular mechanisms of KiSS1 in human OS. The expression of KiSS1 was assessed by immunohistochemical assay using a tissue microarray procedure in forty cases of OS tissues. A gain-of-function approach was used to observe the effects of lentiviral vector-mediated overexpression of KiSS1 (Lv-KiSS1) on the biological behaviors including proliferative activities and invasive potential of OS MG-63 cells, indicated by MTT and Transwell assays, respectively. The results showed that the expression of KiSS1 protein in OS tissues was significantly lowered compared to that in adjacent non-cancerous tissues (42.5% vs 70.0%, P=0.023), and had negative correlation with distant metastases of the tumor (P=0.019). Overexpression of KiSS1 inhibited proliferation and invasion of OS cells with the decreased expression of p38 MAPK and matrix metalloproteinase-9 (MMP-9). Taken together, our findings indicate that the decreased expression of KiSS1 is correlated with distant metastasis of OS, and KiSS1 may function as a tumor suppressor in OS cells through inhibition of the MAPK pathway, suggesting that KiSS1 may serve as a potential therapeutic target for the treatment of cancer. PAGEPress Publications, Pavia, Italy 2013-10-29 /pmc/articles/PMC3896032/ /pubmed/24441183 http://dx.doi.org/10.4081/ejh.2013.e30 Text en ©Copyright Y. Zhang et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Zhang, Y.
Tang, Y.J.
Li, Z.H.
Pan, F.
Huang, K.
Xu, G.H.
KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title_full KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title_fullStr KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title_full_unstemmed KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title_short KiSS1 Inhibits Growth and Invasion of Osteosarcoma Cells through Inhibition of the MAPK Pathway
title_sort kiss1 inhibits growth and invasion of osteosarcoma cells through inhibition of the mapk pathway
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896032/
https://www.ncbi.nlm.nih.gov/pubmed/24441183
http://dx.doi.org/10.4081/ejh.2013.e30
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