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Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats

While the concept of the Neurovascular Unit (NVU) is increasingly considered for exploring mechanisms of tissue damage in ischemic stroke, immunohistochemical analyses are of interest to specifically visualize constituents like the endothelium. Changes in immunoreactivity have also been discussed to...

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Autores principales: Pelz, J., Härtig, W., Weise, C., Hobohm, C., Schneider, D., Krueger, M., Kacza, J., Michalski, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896040/
https://www.ncbi.nlm.nih.gov/pubmed/24441191
http://dx.doi.org/10.4081/ejh.2013.e38
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author Pelz, J.
Härtig, W.
Weise, C.
Hobohm, C.
Schneider, D.
Krueger, M.
Kacza, J.
Michalski, D.
author_facet Pelz, J.
Härtig, W.
Weise, C.
Hobohm, C.
Schneider, D.
Krueger, M.
Kacza, J.
Michalski, D.
author_sort Pelz, J.
collection PubMed
description While the concept of the Neurovascular Unit (NVU) is increasingly considered for exploring mechanisms of tissue damage in ischemic stroke, immunohistochemical analyses are of interest to specifically visualize constituents like the endothelium. Changes in immunoreactivity have also been discussed to reflect functional aspects, e.g., the integrity of the blood-brain barrier (BBB). This study aimed to characterize the endothelial barrier antigen (EBA) as addressed by the antibody SMI-71 in a rat model of embolic stroke, considering FITC-albumin as BBB leakage marker and serum levels of BBB-associated matrix metalloproteinases (MMPs) to explore its functional significance. Five and 25 h after ischemia onset, regions with decreased BBB integrity exhibited a reduction in number and area of EBA-immunopositive vessels, while the stained area per vessel was not affected. Surprisingly, EBA content of remaining vessels tended to be increased in areas of BBB dysfunction. Analyses addressing this interrelation resulted in a significant and inverse correlation between the vessels’ EBA content and degree of BBB permeability. In conclusion, these data provide evidence for a functional relationship between EBA-immunoreactivity and BBB dysfunction in experimental ischemic stroke. Further studies are required to explore the underlying mechanisms of altered EBA-immunoreactivity, which might help to identify novel neuroprotective strategies.
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spelling pubmed-38960402014-01-24 Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats Pelz, J. Härtig, W. Weise, C. Hobohm, C. Schneider, D. Krueger, M. Kacza, J. Michalski, D. Eur J Histochem Original Paper While the concept of the Neurovascular Unit (NVU) is increasingly considered for exploring mechanisms of tissue damage in ischemic stroke, immunohistochemical analyses are of interest to specifically visualize constituents like the endothelium. Changes in immunoreactivity have also been discussed to reflect functional aspects, e.g., the integrity of the blood-brain barrier (BBB). This study aimed to characterize the endothelial barrier antigen (EBA) as addressed by the antibody SMI-71 in a rat model of embolic stroke, considering FITC-albumin as BBB leakage marker and serum levels of BBB-associated matrix metalloproteinases (MMPs) to explore its functional significance. Five and 25 h after ischemia onset, regions with decreased BBB integrity exhibited a reduction in number and area of EBA-immunopositive vessels, while the stained area per vessel was not affected. Surprisingly, EBA content of remaining vessels tended to be increased in areas of BBB dysfunction. Analyses addressing this interrelation resulted in a significant and inverse correlation between the vessels’ EBA content and degree of BBB permeability. In conclusion, these data provide evidence for a functional relationship between EBA-immunoreactivity and BBB dysfunction in experimental ischemic stroke. Further studies are required to explore the underlying mechanisms of altered EBA-immunoreactivity, which might help to identify novel neuroprotective strategies. PAGEPress Publications, Pavia, Italy 2013-12-18 /pmc/articles/PMC3896040/ /pubmed/24441191 http://dx.doi.org/10.4081/ejh.2013.e38 Text en ©Copyright J. Pelz et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Pelz, J.
Härtig, W.
Weise, C.
Hobohm, C.
Schneider, D.
Krueger, M.
Kacza, J.
Michalski, D.
Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title_full Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title_fullStr Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title_full_unstemmed Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title_short Endothelial Barrier Antigenimmunoreactivity is Conversely Associated with Blood-Brain Barrier Dysfunction after Embolic Stroke in Rats
title_sort endothelial barrier antigenimmunoreactivity is conversely associated with blood-brain barrier dysfunction after embolic stroke in rats
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896040/
https://www.ncbi.nlm.nih.gov/pubmed/24441191
http://dx.doi.org/10.4081/ejh.2013.e38
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