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Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay

BACKGROUND AND PURPOSE OF THE STUDY: Angiotensin converting enzyme (ACE) inhibitors plays a critical role in treating hypertension. The purpose of the present investigation was to evaluate ACE inhibition activity of 50 Iranian medicinal plants using an in vitro assay. METHODS: The ACE activity was e...

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Autores principales: Sharifi, Niusha, Souri, Effat, Ziai, Seyed Ali, Amin, Gholamreza, Amanlou, Massoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896793/
https://www.ncbi.nlm.nih.gov/pubmed/24359711
http://dx.doi.org/10.1186/2008-2231-21-74
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author Sharifi, Niusha
Souri, Effat
Ziai, Seyed Ali
Amin, Gholamreza
Amanlou, Massoud
author_facet Sharifi, Niusha
Souri, Effat
Ziai, Seyed Ali
Amin, Gholamreza
Amanlou, Massoud
author_sort Sharifi, Niusha
collection PubMed
description BACKGROUND AND PURPOSE OF THE STUDY: Angiotensin converting enzyme (ACE) inhibitors plays a critical role in treating hypertension. The purpose of the present investigation was to evaluate ACE inhibition activity of 50 Iranian medicinal plants using an in vitro assay. METHODS: The ACE activity was evaluated by determining the hydrolysis rate of substrate, hippuryl-L-histidyl-L-leucine (HHL), using reverse phase high performance liquid chromatography (RP-HPLC). Total phenolic content and antioxidant activity were determined by Folin-Ciocalteu colorimetric method and DPPH radical scavenging assay respectively. RESULTS: Six extracts revealed > 50% ACE inhibition activity at 330 μg/ml concentration. They were Berberis integerrima Bunge. (Berberidaceae) (88.2 ± 1.7%), Crataegus microphylla C. Koch (Rosaceae) (80.9 ± 1.3%), Nymphaea alba L. (Nymphaeaceae) (66.3 ± 1.2%), Onopordon acanthium L. (Asteraceae) (80.2 ± 2.0%), Quercus infectoria G. Olivier. (Fagaceae) (93.9 ± 2.5%) and Rubus sp. (Rosaceae) (51.3 ± 1.0%). Q. infectoria possessed the highest total phenolic content with 7410 ± 101 mg gallic acid/100 g dry plant. Antioxidant activity of Q. infectoria (IC(50) value 1.7 ± 0.03 μg/ml) was more than that of BHT (IC(50) value of 10.3 ± 0.15 μg/ml) and Trolox (IC(50) value of 3.2 ± 0.06 μg/ml) as the positive controls. CONCLUSIONS: In this study, we introduced six medicinal plants with ACE inhibition activity. Despite the high ACE inhibition and antioxidant activity of Q. infectoria, due to its tannin content (tannins interfere in ACE activity), another plant, O. acanthium, which also had high ACE inhibition and antioxidant activity, but contained no tannin, could be utilized in further studies for isolation of active compounds.
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spelling pubmed-38967932014-01-31 Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay Sharifi, Niusha Souri, Effat Ziai, Seyed Ali Amin, Gholamreza Amanlou, Massoud Daru Research Article BACKGROUND AND PURPOSE OF THE STUDY: Angiotensin converting enzyme (ACE) inhibitors plays a critical role in treating hypertension. The purpose of the present investigation was to evaluate ACE inhibition activity of 50 Iranian medicinal plants using an in vitro assay. METHODS: The ACE activity was evaluated by determining the hydrolysis rate of substrate, hippuryl-L-histidyl-L-leucine (HHL), using reverse phase high performance liquid chromatography (RP-HPLC). Total phenolic content and antioxidant activity were determined by Folin-Ciocalteu colorimetric method and DPPH radical scavenging assay respectively. RESULTS: Six extracts revealed > 50% ACE inhibition activity at 330 μg/ml concentration. They were Berberis integerrima Bunge. (Berberidaceae) (88.2 ± 1.7%), Crataegus microphylla C. Koch (Rosaceae) (80.9 ± 1.3%), Nymphaea alba L. (Nymphaeaceae) (66.3 ± 1.2%), Onopordon acanthium L. (Asteraceae) (80.2 ± 2.0%), Quercus infectoria G. Olivier. (Fagaceae) (93.9 ± 2.5%) and Rubus sp. (Rosaceae) (51.3 ± 1.0%). Q. infectoria possessed the highest total phenolic content with 7410 ± 101 mg gallic acid/100 g dry plant. Antioxidant activity of Q. infectoria (IC(50) value 1.7 ± 0.03 μg/ml) was more than that of BHT (IC(50) value of 10.3 ± 0.15 μg/ml) and Trolox (IC(50) value of 3.2 ± 0.06 μg/ml) as the positive controls. CONCLUSIONS: In this study, we introduced six medicinal plants with ACE inhibition activity. Despite the high ACE inhibition and antioxidant activity of Q. infectoria, due to its tannin content (tannins interfere in ACE activity), another plant, O. acanthium, which also had high ACE inhibition and antioxidant activity, but contained no tannin, could be utilized in further studies for isolation of active compounds. BioMed Central 2013-12-20 /pmc/articles/PMC3896793/ /pubmed/24359711 http://dx.doi.org/10.1186/2008-2231-21-74 Text en Copyright © 2013 Sharifi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sharifi, Niusha
Souri, Effat
Ziai, Seyed Ali
Amin, Gholamreza
Amanlou, Massoud
Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title_full Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title_fullStr Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title_full_unstemmed Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title_short Discovery of new angiotensin converting enzyme (ACE) inhibitors from medicinal plants to treat hypertension using an in vitro assay
title_sort discovery of new angiotensin converting enzyme (ace) inhibitors from medicinal plants to treat hypertension using an in vitro assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3896793/
https://www.ncbi.nlm.nih.gov/pubmed/24359711
http://dx.doi.org/10.1186/2008-2231-21-74
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