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Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA
BACKGROUND: Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) such as eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) are critical for human health and development. Numerous studies have indicated that deficiencies in these fatty acids can increase the risk or severity o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897407/ https://www.ncbi.nlm.nih.gov/pubmed/24465476 http://dx.doi.org/10.1371/journal.pone.0085061 |
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author | Petrie, James R. Shrestha, Pushkar Belide, Srinivas Kennedy, Yoko Lester, Geraldine Liu, Qing Divi, Uday K. Mulder, Roger J. Mansour, Maged P. Nichols, Peter D. Singh, Surinder P. |
author_facet | Petrie, James R. Shrestha, Pushkar Belide, Srinivas Kennedy, Yoko Lester, Geraldine Liu, Qing Divi, Uday K. Mulder, Roger J. Mansour, Maged P. Nichols, Peter D. Singh, Surinder P. |
author_sort | Petrie, James R. |
collection | PubMed |
description | BACKGROUND: Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) such as eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) are critical for human health and development. Numerous studies have indicated that deficiencies in these fatty acids can increase the risk or severity of cardiovascular, inflammatory and other diseases or disorders. EPA and DHA are predominantly sourced from marine fish although the primary producers are microalgae. Much work has been done to engineer a sustainable land-based source of EPA and DHA to reduce pressure on fish stocks in meeting future demand, with previous studies describing the production of fish oil-like levels of DHA in the model plant species, Arabidopsis thaliana. PRINCIPAL FINDINGS: In this study we describe the production of fish oil-like levels (>12%) of DHA in the oilseed crop species Camelina sativa achieving a high ω3/ω6 ratio. The construct previously transformed in Arabidopsis as well as two modified construct versions designed to increase DHA production were used. DHA was found to be stable to at least the T(5) generation and the EPA and DHA were found to be predominantly at the sn-1,3 positions of triacylglycerols. Transgenic and parental lines did not have different germination or seedling establishment rates. CONCLUSIONS: DHA can be produced at fish oil-like levels in industrially-relevant oilseed crop species using multi-gene construct designs which are stable over multiple generations. This study has implications for the future of sustainable EPA and DHA production from land-based sources. |
format | Online Article Text |
id | pubmed-3897407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38974072014-01-24 Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA Petrie, James R. Shrestha, Pushkar Belide, Srinivas Kennedy, Yoko Lester, Geraldine Liu, Qing Divi, Uday K. Mulder, Roger J. Mansour, Maged P. Nichols, Peter D. Singh, Surinder P. PLoS One Research Article BACKGROUND: Omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) such as eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) are critical for human health and development. Numerous studies have indicated that deficiencies in these fatty acids can increase the risk or severity of cardiovascular, inflammatory and other diseases or disorders. EPA and DHA are predominantly sourced from marine fish although the primary producers are microalgae. Much work has been done to engineer a sustainable land-based source of EPA and DHA to reduce pressure on fish stocks in meeting future demand, with previous studies describing the production of fish oil-like levels of DHA in the model plant species, Arabidopsis thaliana. PRINCIPAL FINDINGS: In this study we describe the production of fish oil-like levels (>12%) of DHA in the oilseed crop species Camelina sativa achieving a high ω3/ω6 ratio. The construct previously transformed in Arabidopsis as well as two modified construct versions designed to increase DHA production were used. DHA was found to be stable to at least the T(5) generation and the EPA and DHA were found to be predominantly at the sn-1,3 positions of triacylglycerols. Transgenic and parental lines did not have different germination or seedling establishment rates. CONCLUSIONS: DHA can be produced at fish oil-like levels in industrially-relevant oilseed crop species using multi-gene construct designs which are stable over multiple generations. This study has implications for the future of sustainable EPA and DHA production from land-based sources. Public Library of Science 2014-01-21 /pmc/articles/PMC3897407/ /pubmed/24465476 http://dx.doi.org/10.1371/journal.pone.0085061 Text en © 2014 Petrie et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Petrie, James R. Shrestha, Pushkar Belide, Srinivas Kennedy, Yoko Lester, Geraldine Liu, Qing Divi, Uday K. Mulder, Roger J. Mansour, Maged P. Nichols, Peter D. Singh, Surinder P. Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title | Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title_full | Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title_fullStr | Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title_full_unstemmed | Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title_short | Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA |
title_sort | metabolic engineering camelina sativa with fish oil-like levels of dha |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897407/ https://www.ncbi.nlm.nih.gov/pubmed/24465476 http://dx.doi.org/10.1371/journal.pone.0085061 |
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