Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma

MicroRNA-214 (MiR-214) is aberrantly expressed in several human tumors such as ovarian cancer and breast cancer. However, the role of miR-214 in nasopharyngeal carcinoma (NPC) is still unknown. In this study, we report that miR-214 was overexpressed in NPC cell lines and tissues. Silencing of miR-21...

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Autores principales: Zhang, Zi-Chen, Li, Yang-Yang, Wang, Hai-Yun, Fu, Sha, Wang, Xiao-Pai, Zeng, Mu-Sheng, Zeng, Yi-Xin, Shao, Jian-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897649/
https://www.ncbi.nlm.nih.gov/pubmed/24465927
http://dx.doi.org/10.1371/journal.pone.0086149
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author Zhang, Zi-Chen
Li, Yang-Yang
Wang, Hai-Yun
Fu, Sha
Wang, Xiao-Pai
Zeng, Mu-Sheng
Zeng, Yi-Xin
Shao, Jian-Yong
author_facet Zhang, Zi-Chen
Li, Yang-Yang
Wang, Hai-Yun
Fu, Sha
Wang, Xiao-Pai
Zeng, Mu-Sheng
Zeng, Yi-Xin
Shao, Jian-Yong
author_sort Zhang, Zi-Chen
collection PubMed
description MicroRNA-214 (MiR-214) is aberrantly expressed in several human tumors such as ovarian cancer and breast cancer. However, the role of miR-214 in nasopharyngeal carcinoma (NPC) is still unknown. In this study, we report that miR-214 was overexpressed in NPC cell lines and tissues. Silencing of miR-214 by LNA-antimiR-214 in NPC cells resulted in promoting apoptosis and suppressing cell proliferation in vitro, and suppressed tumor growth in nude mice in vivo. Luciferase reporter assay was performed to identify Bim as a direct target of miR-214. Furthermore, this study showed that low Bim expression in NPC tissues correlated with poor survival of NPC patients. Taken together, our findings suggest that miR-214 plays an important role in NPC carcinogenesis.
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spelling pubmed-38976492014-01-24 Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma Zhang, Zi-Chen Li, Yang-Yang Wang, Hai-Yun Fu, Sha Wang, Xiao-Pai Zeng, Mu-Sheng Zeng, Yi-Xin Shao, Jian-Yong PLoS One Research Article MicroRNA-214 (MiR-214) is aberrantly expressed in several human tumors such as ovarian cancer and breast cancer. However, the role of miR-214 in nasopharyngeal carcinoma (NPC) is still unknown. In this study, we report that miR-214 was overexpressed in NPC cell lines and tissues. Silencing of miR-214 by LNA-antimiR-214 in NPC cells resulted in promoting apoptosis and suppressing cell proliferation in vitro, and suppressed tumor growth in nude mice in vivo. Luciferase reporter assay was performed to identify Bim as a direct target of miR-214. Furthermore, this study showed that low Bim expression in NPC tissues correlated with poor survival of NPC patients. Taken together, our findings suggest that miR-214 plays an important role in NPC carcinogenesis. Public Library of Science 2014-01-21 /pmc/articles/PMC3897649/ /pubmed/24465927 http://dx.doi.org/10.1371/journal.pone.0086149 Text en © 2014 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Zi-Chen
Li, Yang-Yang
Wang, Hai-Yun
Fu, Sha
Wang, Xiao-Pai
Zeng, Mu-Sheng
Zeng, Yi-Xin
Shao, Jian-Yong
Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title_full Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title_fullStr Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title_full_unstemmed Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title_short Knockdown of miR-214 Promotes Apoptosis and Inhibits Cell Proliferation in Nasopharyngeal Carcinoma
title_sort knockdown of mir-214 promotes apoptosis and inhibits cell proliferation in nasopharyngeal carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897649/
https://www.ncbi.nlm.nih.gov/pubmed/24465927
http://dx.doi.org/10.1371/journal.pone.0086149
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