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In vivo biofunctional evaluation of hydrogels for disc regeneration

PURPOSE: Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seed...

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Autores principales: Reitmaier, Sandra, Kreja, Ludwika, Gruchenberg, Katharina, Kanter, Britta, Silva-Correia, Joana, Oliveira, Joaquim Miguel, Reis, Rui Luís, Perugini, Valeria, Santin, Matteo, Ignatius, Anita, Wilke, Hans-Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897837/
https://www.ncbi.nlm.nih.gov/pubmed/24121748
http://dx.doi.org/10.1007/s00586-013-2998-8
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author Reitmaier, Sandra
Kreja, Ludwika
Gruchenberg, Katharina
Kanter, Britta
Silva-Correia, Joana
Oliveira, Joaquim Miguel
Reis, Rui Luís
Perugini, Valeria
Santin, Matteo
Ignatius, Anita
Wilke, Hans-Joachim
author_facet Reitmaier, Sandra
Kreja, Ludwika
Gruchenberg, Katharina
Kanter, Britta
Silva-Correia, Joana
Oliveira, Joaquim Miguel
Reis, Rui Luís
Perugini, Valeria
Santin, Matteo
Ignatius, Anita
Wilke, Hans-Joachim
author_sort Reitmaier, Sandra
collection PubMed
description PURPOSE: Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. METHODS: In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. RESULTS: Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. CONCLUSIONS: None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioning.
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spelling pubmed-38978372014-01-24 In vivo biofunctional evaluation of hydrogels for disc regeneration Reitmaier, Sandra Kreja, Ludwika Gruchenberg, Katharina Kanter, Britta Silva-Correia, Joana Oliveira, Joaquim Miguel Reis, Rui Luís Perugini, Valeria Santin, Matteo Ignatius, Anita Wilke, Hans-Joachim Eur Spine J EUROSPINE 2013 Full Paper Award PURPOSE: Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. METHODS: In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. RESULTS: Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. CONCLUSIONS: None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioning. Springer Berlin Heidelberg 2013-10-12 2014-01 /pmc/articles/PMC3897837/ /pubmed/24121748 http://dx.doi.org/10.1007/s00586-013-2998-8 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle EUROSPINE 2013 Full Paper Award
Reitmaier, Sandra
Kreja, Ludwika
Gruchenberg, Katharina
Kanter, Britta
Silva-Correia, Joana
Oliveira, Joaquim Miguel
Reis, Rui Luís
Perugini, Valeria
Santin, Matteo
Ignatius, Anita
Wilke, Hans-Joachim
In vivo biofunctional evaluation of hydrogels for disc regeneration
title In vivo biofunctional evaluation of hydrogels for disc regeneration
title_full In vivo biofunctional evaluation of hydrogels for disc regeneration
title_fullStr In vivo biofunctional evaluation of hydrogels for disc regeneration
title_full_unstemmed In vivo biofunctional evaluation of hydrogels for disc regeneration
title_short In vivo biofunctional evaluation of hydrogels for disc regeneration
title_sort in vivo biofunctional evaluation of hydrogels for disc regeneration
topic EUROSPINE 2013 Full Paper Award
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897837/
https://www.ncbi.nlm.nih.gov/pubmed/24121748
http://dx.doi.org/10.1007/s00586-013-2998-8
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