Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coli and preliminary purification process

BACKGROUND: Streptococcus pneumoniae (S. pneumoniae) causes several serious diseases including pneumonia, septicemia and meningitis. The World Health Organization estimates that streptococcal pneumonia is the cause of approximately 1.9 million deaths of children under five years of age each year. Th...

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Detalles Bibliográficos
Autores principales: Marini, Guillermo, Luchese, Mateus Dalcin, Argondizzo, Ana Paula Correa, de Góes, Ana Carolina Magalhães Andrade, Galler, Ricardo, Alves, Tito Lívio Moitinho, Medeiros, Marco Alberto, Larentis, Ariane Leites
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897902/
https://www.ncbi.nlm.nih.gov/pubmed/24400649
http://dx.doi.org/10.1186/1472-6750-14-1

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