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Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are s...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897965/ https://www.ncbi.nlm.nih.gov/pubmed/24410930 http://dx.doi.org/10.1186/1475-925X-13-2 |
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author | Dong, Rong Long, Jinhua Xu, Xiaoli Zhang, Chunlin Wen, Zongyao Li, Long Yao, Weijuan Zeng, Zhu |
author_facet | Dong, Rong Long, Jinhua Xu, Xiaoli Zhang, Chunlin Wen, Zongyao Li, Long Yao, Weijuan Zeng, Zhu |
author_sort | Dong, Rong |
collection | PubMed |
description | BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states. METHODS: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods. RESULTS: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R(2):0.69 and R(2):0.81, respectively). CONCLUSION: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols. |
format | Online Article Text |
id | pubmed-3897965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38979652014-01-23 Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy Dong, Rong Long, Jinhua Xu, Xiaoli Zhang, Chunlin Wen, Zongyao Li, Long Yao, Weijuan Zeng, Zhu Biomed Eng Online Research BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states. METHODS: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods. RESULTS: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R(2):0.69 and R(2):0.81, respectively). CONCLUSION: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols. BioMed Central 2014-01-10 /pmc/articles/PMC3897965/ /pubmed/24410930 http://dx.doi.org/10.1186/1475-925X-13-2 Text en Copyright © 2014 Dong et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Dong, Rong Long, Jinhua Xu, Xiaoli Zhang, Chunlin Wen, Zongyao Li, Long Yao, Weijuan Zeng, Zhu Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title | Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title_full | Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title_fullStr | Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title_full_unstemmed | Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title_short | Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy |
title_sort | investigations of the functional states of dendritic cells under different conditioned microenvironments by fourier transformed infrared spectroscopy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897965/ https://www.ncbi.nlm.nih.gov/pubmed/24410930 http://dx.doi.org/10.1186/1475-925X-13-2 |
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