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Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy

BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are s...

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Autores principales: Dong, Rong, Long, Jinhua, Xu, Xiaoli, Zhang, Chunlin, Wen, Zongyao, Li, Long, Yao, Weijuan, Zeng, Zhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897965/
https://www.ncbi.nlm.nih.gov/pubmed/24410930
http://dx.doi.org/10.1186/1475-925X-13-2
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author Dong, Rong
Long, Jinhua
Xu, Xiaoli
Zhang, Chunlin
Wen, Zongyao
Li, Long
Yao, Weijuan
Zeng, Zhu
author_facet Dong, Rong
Long, Jinhua
Xu, Xiaoli
Zhang, Chunlin
Wen, Zongyao
Li, Long
Yao, Weijuan
Zeng, Zhu
author_sort Dong, Rong
collection PubMed
description BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states. METHODS: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods. RESULTS: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R(2):0.69 and R(2):0.81, respectively). CONCLUSION: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.
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spelling pubmed-38979652014-01-23 Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy Dong, Rong Long, Jinhua Xu, Xiaoli Zhang, Chunlin Wen, Zongyao Li, Long Yao, Weijuan Zeng, Zhu Biomed Eng Online Research BACKGROUND: Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states. METHODS: The CD14(+) monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods. RESULTS: The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P < 0.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P < 0.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R(2):0.69 and R(2):0.81, respectively). CONCLUSION: Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols. BioMed Central 2014-01-10 /pmc/articles/PMC3897965/ /pubmed/24410930 http://dx.doi.org/10.1186/1475-925X-13-2 Text en Copyright © 2014 Dong et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Dong, Rong
Long, Jinhua
Xu, Xiaoli
Zhang, Chunlin
Wen, Zongyao
Li, Long
Yao, Weijuan
Zeng, Zhu
Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title_full Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title_fullStr Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title_full_unstemmed Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title_short Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy
title_sort investigations of the functional states of dendritic cells under different conditioned microenvironments by fourier transformed infrared spectroscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3897965/
https://www.ncbi.nlm.nih.gov/pubmed/24410930
http://dx.doi.org/10.1186/1475-925X-13-2
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