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A novel estrogen-regulated avian apolipoprotein()
In search for yet uncharacterized proteins involved in lipid metabolism of the chicken, we have isolated a hitherto unknown protein from the serum lipoprotein fraction with a buoyant density of ≤1.063 g/ml. Data obtained by protein microsequencing and molecular cloning of cDNA defined a 537 bp cDNA...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editions Scientifiques Elsevier
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898076/ https://www.ncbi.nlm.nih.gov/pubmed/24047540 http://dx.doi.org/10.1016/j.biochi.2013.09.005 |
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author | Nikolay, Birgit Plieschnig, Julia A. Šubik, Desiree Schneider, Jeannine D. Schneider, Wolfgang J. Hermann, Marcela |
author_facet | Nikolay, Birgit Plieschnig, Julia A. Šubik, Desiree Schneider, Jeannine D. Schneider, Wolfgang J. Hermann, Marcela |
author_sort | Nikolay, Birgit |
collection | PubMed |
description | In search for yet uncharacterized proteins involved in lipid metabolism of the chicken, we have isolated a hitherto unknown protein from the serum lipoprotein fraction with a buoyant density of ≤1.063 g/ml. Data obtained by protein microsequencing and molecular cloning of cDNA defined a 537 bp cDNA encoding a precursor molecule of 178 residues. As determined by SDS-PAGE, the major circulating form of the protein, which we designate apolipoprotein-VLDL-IV (Apo-IV), has an apparent M(r) of approximately 17 kDa. Northern Blot analysis of different tissues of laying hens revealed Apo-IV expression mainly in the liver and small intestine, compatible with an involvement of the protein in lipoprotein metabolism. To further investigate the biology of Apo-IV, we raised an antibody against a GST-Apo-IV fusion protein, which allowed the detection of the 17-kDa protein in rooster plasma, whereas in laying hens it was detectable only in the isolated ≤1.063 g/ml density lipoprotein fraction. Interestingly, estrogen treatment of roosters caused a reduction of Apo-IV in the liver and in the circulation to levels similar to those in mature hens. Furthermore, the antibody crossreacted with a 17-kDa protein in quail plasma, indicating conservation of Apo-IV in avian species. In search for mammalian counterparts of Apo-IV, alignment of the sequence of the novel chicken protein with those of different mammalian apolipoproteins revealed stretches with limited similarity to regions of ApoC-IV and possibly with ApoE from various mammalian species. These data suggest that Apo-IV is a newly identified avian apolipoprotein. |
format | Online Article Text |
id | pubmed-3898076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Editions Scientifiques Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-38980762014-01-24 A novel estrogen-regulated avian apolipoprotein() Nikolay, Birgit Plieschnig, Julia A. Šubik, Desiree Schneider, Jeannine D. Schneider, Wolfgang J. Hermann, Marcela Biochimie Research Paper In search for yet uncharacterized proteins involved in lipid metabolism of the chicken, we have isolated a hitherto unknown protein from the serum lipoprotein fraction with a buoyant density of ≤1.063 g/ml. Data obtained by protein microsequencing and molecular cloning of cDNA defined a 537 bp cDNA encoding a precursor molecule of 178 residues. As determined by SDS-PAGE, the major circulating form of the protein, which we designate apolipoprotein-VLDL-IV (Apo-IV), has an apparent M(r) of approximately 17 kDa. Northern Blot analysis of different tissues of laying hens revealed Apo-IV expression mainly in the liver and small intestine, compatible with an involvement of the protein in lipoprotein metabolism. To further investigate the biology of Apo-IV, we raised an antibody against a GST-Apo-IV fusion protein, which allowed the detection of the 17-kDa protein in rooster plasma, whereas in laying hens it was detectable only in the isolated ≤1.063 g/ml density lipoprotein fraction. Interestingly, estrogen treatment of roosters caused a reduction of Apo-IV in the liver and in the circulation to levels similar to those in mature hens. Furthermore, the antibody crossreacted with a 17-kDa protein in quail plasma, indicating conservation of Apo-IV in avian species. In search for mammalian counterparts of Apo-IV, alignment of the sequence of the novel chicken protein with those of different mammalian apolipoproteins revealed stretches with limited similarity to regions of ApoC-IV and possibly with ApoE from various mammalian species. These data suggest that Apo-IV is a newly identified avian apolipoprotein. Editions Scientifiques Elsevier 2013-12 /pmc/articles/PMC3898076/ /pubmed/24047540 http://dx.doi.org/10.1016/j.biochi.2013.09.005 Text en © 2013 The Authors https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license |
spellingShingle | Research Paper Nikolay, Birgit Plieschnig, Julia A. Šubik, Desiree Schneider, Jeannine D. Schneider, Wolfgang J. Hermann, Marcela A novel estrogen-regulated avian apolipoprotein() |
title | A novel estrogen-regulated avian apolipoprotein() |
title_full | A novel estrogen-regulated avian apolipoprotein() |
title_fullStr | A novel estrogen-regulated avian apolipoprotein() |
title_full_unstemmed | A novel estrogen-regulated avian apolipoprotein() |
title_short | A novel estrogen-regulated avian apolipoprotein() |
title_sort | novel estrogen-regulated avian apolipoprotein() |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898076/ https://www.ncbi.nlm.nih.gov/pubmed/24047540 http://dx.doi.org/10.1016/j.biochi.2013.09.005 |
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