Cargando…
Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care
HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing co...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898414/ https://www.ncbi.nlm.nih.gov/pubmed/24448112 http://dx.doi.org/10.1038/srep03796 |
_version_ | 1782300419823763456 |
---|---|
author | Wang, ShuQi Tasoglu, Savas Chen, Paul Z. Chen, Michael Akbas, Ragip Wach, Sonya Ozdemir, Cenk Ibrahim Gurkan, Umut Atakan Giguel, Francoise F. Kuritzkes, Daniel R. Demirci, Utkan |
author_facet | Wang, ShuQi Tasoglu, Savas Chen, Paul Z. Chen, Michael Akbas, Ragip Wach, Sonya Ozdemir, Cenk Ibrahim Gurkan, Umut Atakan Giguel, Francoise F. Kuritzkes, Daniel R. Demirci, Utkan |
author_sort | Wang, ShuQi |
collection | PubMed |
description | HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays. |
format | Online Article Text |
id | pubmed-3898414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-38984142014-01-24 Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care Wang, ShuQi Tasoglu, Savas Chen, Paul Z. Chen, Michael Akbas, Ragip Wach, Sonya Ozdemir, Cenk Ibrahim Gurkan, Umut Atakan Giguel, Francoise F. Kuritzkes, Daniel R. Demirci, Utkan Sci Rep Article HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays. Nature Publishing Group 2014-01-22 /pmc/articles/PMC3898414/ /pubmed/24448112 http://dx.doi.org/10.1038/srep03796 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Wang, ShuQi Tasoglu, Savas Chen, Paul Z. Chen, Michael Akbas, Ragip Wach, Sonya Ozdemir, Cenk Ibrahim Gurkan, Umut Atakan Giguel, Francoise F. Kuritzkes, Daniel R. Demirci, Utkan Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title_full | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title_fullStr | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title_full_unstemmed | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title_short | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
title_sort | micro-a-fluidics elisa for rapid cd4 cell count at the point-of-care |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898414/ https://www.ncbi.nlm.nih.gov/pubmed/24448112 http://dx.doi.org/10.1038/srep03796 |
work_keys_str_mv | AT wangshuqi microafluidicselisaforrapidcd4cellcountatthepointofcare AT tasoglusavas microafluidicselisaforrapidcd4cellcountatthepointofcare AT chenpaulz microafluidicselisaforrapidcd4cellcountatthepointofcare AT chenmichael microafluidicselisaforrapidcd4cellcountatthepointofcare AT akbasragip microafluidicselisaforrapidcd4cellcountatthepointofcare AT wachsonya microafluidicselisaforrapidcd4cellcountatthepointofcare AT ozdemircenkibrahim microafluidicselisaforrapidcd4cellcountatthepointofcare AT gurkanumutatakan microafluidicselisaforrapidcd4cellcountatthepointofcare AT giguelfrancoisef microafluidicselisaforrapidcd4cellcountatthepointofcare AT kuritzkesdanielr microafluidicselisaforrapidcd4cellcountatthepointofcare AT demirciutkan microafluidicselisaforrapidcd4cellcountatthepointofcare |