Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering

Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of...

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Detalles Bibliográficos
Autores principales: Antoun, Ayman, Pavlov, Michael Y., Tenson, Tanel, Ehrenberg, Måns
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389903/
https://www.ncbi.nlm.nih.gov/pubmed/15103398
http://dx.doi.org/10.1251/bpo71
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author Antoun, Ayman
Pavlov, Michael Y.
Tenson, Tanel
Ehrenberg, Måns
author_facet Antoun, Ayman
Pavlov, Michael Y.
Tenson, Tanel
Ehrenberg, Måns
author_sort Antoun, Ayman
collection PubMed
description Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is high-lighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix.
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spelling pubmed-3899032004-04-20 Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering Antoun, Ayman Pavlov, Michael Y. Tenson, Tanel Ehrenberg, Måns Biol Proced Online Research Article Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is high-lighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix. Biological Procedures Online 2004-03-19 /pmc/articles/PMC389903/ /pubmed/15103398 http://dx.doi.org/10.1251/bpo71 Text en Copyright © March 03, 2004, A Antoun et al. Published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted.
spellingShingle Research Article
Antoun, Ayman
Pavlov, Michael Y.
Tenson, Tanel
Ehrenberg, Måns
Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title_full Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title_fullStr Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title_full_unstemmed Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title_short Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering
title_sort ribosome formation from subunits studied by stopped-flow and rayleigh light scattering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC389903/
https://www.ncbi.nlm.nih.gov/pubmed/15103398
http://dx.doi.org/10.1251/bpo71
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AT pavlovmichaely ribosomeformationfromsubunitsstudiedbystoppedflowandrayleighlightscattering
AT tensontanel ribosomeformationfromsubunitsstudiedbystoppedflowandrayleighlightscattering
AT ehrenbergmans ribosomeformationfromsubunitsstudiedbystoppedflowandrayleighlightscattering

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