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Evaluation of Reference Genes for Accurate Normalization of Gene Expression for Real Time-Quantitative PCR in Pyrus pyrifolia Using Different Tissue Samples and Seasonal Conditions

We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α, Glyceraldehyde-3-phosphate dehydrogenase, together wit...

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Detalles Bibliográficos
Autores principales:	Imai, Tsuyoshi, Ubi, Benjamin E., Saito, Takanori, Moriguchi, Takaya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899261/ https://www.ncbi.nlm.nih.gov/pubmed/24466117 http://dx.doi.org/10.1371/journal.pone.0086492

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