Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue

A one-step enzymatic reaction for improving the collision-induced dissociation (CID)-based tandem mass spectrometry (MS/MS) analysis of phosphorylated peptides in an ion trap is presented. Carboxypeptidase-B (CBP-B) was used to selectively remove C-terminal arginine or lysine residues from phosphory...

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Detalles Bibliográficos
Autores principales: Lanucara, Francesco, Chi Hoo Lee, Dave, Eyers, Claire E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899453/
https://www.ncbi.nlm.nih.gov/pubmed/24297471
http://dx.doi.org/10.1007/s13361-013-0770-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899453/
https://www.ncbi.nlm.nih.gov/pubmed/24297471
http://dx.doi.org/10.1007/s13361-013-0770-2

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