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Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis

BACKGROUND: Nasopharyngeal carcinoma (NPC) has a distinctive geographic distribution and is characterised by its strong tendency of metastasis. We aimed to examine the microRNA (miRNA) expression profiles in plasma samples of NPC patients to explore their clinical significance in disease development...

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Autores principales: Lu, J, Xu, X, Liu, X, Peng, Y, Zhang, B, Wang, L, Luo, H, Peng, X, Li, G, Tian, W, He, M, Li, X
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899774/
https://www.ncbi.nlm.nih.gov/pubmed/24327016
http://dx.doi.org/10.1038/bjc.2013.751
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author Lu, J
Xu, X
Liu, X
Peng, Y
Zhang, B
Wang, L
Luo, H
Peng, X
Li, G
Tian, W
He, M
Li, X
author_facet Lu, J
Xu, X
Liu, X
Peng, Y
Zhang, B
Wang, L
Luo, H
Peng, X
Li, G
Tian, W
He, M
Li, X
author_sort Lu, J
collection PubMed
description BACKGROUND: Nasopharyngeal carcinoma (NPC) has a distinctive geographic distribution and is characterised by its strong tendency of metastasis. We aimed to examine the microRNA (miRNA) expression profiles in plasma samples of NPC patients to explore their clinical significance in disease development and progression. METHODS: This study was divided into four steps: (1) confirmation of differentially expressed miRNAs using microarray analysis and quantitative PCR validation; (2) comparison of plasma miR-9 levels during NPC progression; (3) evaluation of the predictive performance of plasma miR-9 as a biomarker for NPC metastasis; and (4) comparison of plasma miR-9 levels between pre- and post-treatment samples. RESULTS: Plasma microarray profiling identified 33 differentially expressed miRNAs between NPC patients and healthy volunteers. The significantly declined level of miR-9 in NPC patients was confirmed through two-stage validation. The low level of plasma miR-9 was significantly correlated with worse lymphatic invasion and advanced TNM stage. The plasma miR-9 could distinguish locoregional from metastatic NPC cases with a high sensitivity and specificity. Furthermore, the plasma miR-9 level was significantly elevated in post-treatment plasma compared with those pre-treatment samples. CONCLUSION: Our study reports that plasma miR-9 may serve as a useful biomarker to predict NPC metastasis and to monitor tumour dynamics.
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spelling pubmed-38997742015-01-21 Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis Lu, J Xu, X Liu, X Peng, Y Zhang, B Wang, L Luo, H Peng, X Li, G Tian, W He, M Li, X Br J Cancer Molecular Diagnostics BACKGROUND: Nasopharyngeal carcinoma (NPC) has a distinctive geographic distribution and is characterised by its strong tendency of metastasis. We aimed to examine the microRNA (miRNA) expression profiles in plasma samples of NPC patients to explore their clinical significance in disease development and progression. METHODS: This study was divided into four steps: (1) confirmation of differentially expressed miRNAs using microarray analysis and quantitative PCR validation; (2) comparison of plasma miR-9 levels during NPC progression; (3) evaluation of the predictive performance of plasma miR-9 as a biomarker for NPC metastasis; and (4) comparison of plasma miR-9 levels between pre- and post-treatment samples. RESULTS: Plasma microarray profiling identified 33 differentially expressed miRNAs between NPC patients and healthy volunteers. The significantly declined level of miR-9 in NPC patients was confirmed through two-stage validation. The low level of plasma miR-9 was significantly correlated with worse lymphatic invasion and advanced TNM stage. The plasma miR-9 could distinguish locoregional from metastatic NPC cases with a high sensitivity and specificity. Furthermore, the plasma miR-9 level was significantly elevated in post-treatment plasma compared with those pre-treatment samples. CONCLUSION: Our study reports that plasma miR-9 may serve as a useful biomarker to predict NPC metastasis and to monitor tumour dynamics. Nature Publishing Group 2014-01-21 2013-12-10 /pmc/articles/PMC3899774/ /pubmed/24327016 http://dx.doi.org/10.1038/bjc.2013.751 Text en Copyright © 2014 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Molecular Diagnostics
Lu, J
Xu, X
Liu, X
Peng, Y
Zhang, B
Wang, L
Luo, H
Peng, X
Li, G
Tian, W
He, M
Li, X
Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title_full Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title_fullStr Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title_full_unstemmed Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title_short Predictive value of miR-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
title_sort predictive value of mir-9 as a potential biomarker for nasopharyngeal carcinoma metastasis
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899774/
https://www.ncbi.nlm.nih.gov/pubmed/24327016
http://dx.doi.org/10.1038/bjc.2013.751
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