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High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1
Short Interspersed Nuclear Elements (SINEs) are non-autonomous retrotransposons that comprise a large fraction of the human genome. SINEs are demethylated in human disease, but whether SINEs become transcriptionally induced and how the resulting transcripts may affect the expression of protein codin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899967/ https://www.ncbi.nlm.nih.gov/pubmed/24705161 http://dx.doi.org/10.3390/genes4020226 |
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author | Peterson, Maureen Chandler, Vicki L. Bosco, Giovanni |
author_facet | Peterson, Maureen Chandler, Vicki L. Bosco, Giovanni |
author_sort | Peterson, Maureen |
collection | PubMed |
description | Short Interspersed Nuclear Elements (SINEs) are non-autonomous retrotransposons that comprise a large fraction of the human genome. SINEs are demethylated in human disease, but whether SINEs become transcriptionally induced and how the resulting transcripts may affect the expression of protein coding genes is unknown. Here, we show that downregulation of the mRNA of the tumor suppressor gene BRCA1 is associated with increased transcription of SINEs and production of sense and antisense SINE small RNAs. We find that BRCA1 mRNA is post-transcriptionally down-regulated in a Dicer and Drosha dependent manner and that expression of a SINE inverted repeat with sequence identity to a BRCA1 intron is sufficient for downregulation of BRCA1 mRNA. These observations suggest that transcriptional activation of SINEs could contribute to a novel mechanism of RNA mediated post-transcriptional silencing of human genes. |
format | Online Article Text |
id | pubmed-3899967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-38999672014-03-26 High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 Peterson, Maureen Chandler, Vicki L. Bosco, Giovanni Genes (Basel) Article Short Interspersed Nuclear Elements (SINEs) are non-autonomous retrotransposons that comprise a large fraction of the human genome. SINEs are demethylated in human disease, but whether SINEs become transcriptionally induced and how the resulting transcripts may affect the expression of protein coding genes is unknown. Here, we show that downregulation of the mRNA of the tumor suppressor gene BRCA1 is associated with increased transcription of SINEs and production of sense and antisense SINE small RNAs. We find that BRCA1 mRNA is post-transcriptionally down-regulated in a Dicer and Drosha dependent manner and that expression of a SINE inverted repeat with sequence identity to a BRCA1 intron is sufficient for downregulation of BRCA1 mRNA. These observations suggest that transcriptional activation of SINEs could contribute to a novel mechanism of RNA mediated post-transcriptional silencing of human genes. MDPI 2013-04-29 /pmc/articles/PMC3899967/ /pubmed/24705161 http://dx.doi.org/10.3390/genes4020226 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Peterson, Maureen Chandler, Vicki L. Bosco, Giovanni High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title | High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title_full | High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title_fullStr | High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title_full_unstemmed | High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title_short | High SINE RNA Expression Correlates with Post-Transcriptional Downregulation of BRCA1 |
title_sort | high sine rna expression correlates with post-transcriptional downregulation of brca1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899967/ https://www.ncbi.nlm.nih.gov/pubmed/24705161 http://dx.doi.org/10.3390/genes4020226 |
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