Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily

The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the d...

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Detalles Bibliográficos
Autores principales: Reiner, Orly, Gorelik, Anna, Greenman, Raanan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899981/
https://www.ncbi.nlm.nih.gov/pubmed/24705084
http://dx.doi.org/10.3390/genes3040759
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author Reiner, Orly
Gorelik, Anna
Greenman, Raanan
author_facet Reiner, Orly
Gorelik, Anna
Greenman, Raanan
author_sort Reiner, Orly
collection PubMed
description The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins.
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spelling pubmed-38999812014-03-26 Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily Reiner, Orly Gorelik, Anna Greenman, Raanan Genes (Basel) Review The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins. MDPI 2012-11-21 /pmc/articles/PMC3899981/ /pubmed/24705084 http://dx.doi.org/10.3390/genes3040759 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Reiner, Orly
Gorelik, Anna
Greenman, Raanan
Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_full Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_fullStr Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_full_unstemmed Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_short Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_sort use of rna interference by in utero electroporation to study cortical development: the example of the doublecortin superfamily
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3899981/
https://www.ncbi.nlm.nih.gov/pubmed/24705084
http://dx.doi.org/10.3390/genes3040759
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