Cargando…

eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1

BACKGROUND: Hepatitis B virus (HBV) infection and its sequelae are now recognized as serious problems globally. Our aime is to screen hepatocellular carcinoma (HCC) from chronic hepatitis B (CHB) and identify the characteristics of proteins involved. METHODOLOGY/PRINCIPAL FINDINGS: We affinity-purif...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Man, Wang, Jian, Yang, Lei, Gao, Ping, Tian, Qing-bao, Liu, Dian-wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900531/
https://www.ncbi.nlm.nih.gov/pubmed/24466059
http://dx.doi.org/10.1371/journal.pone.0086371
_version_ 1782300712377516032
author Li, Man
Wang, Jian
Yang, Lei
Gao, Ping
Tian, Qing-bao
Liu, Dian-wu
author_facet Li, Man
Wang, Jian
Yang, Lei
Gao, Ping
Tian, Qing-bao
Liu, Dian-wu
author_sort Li, Man
collection PubMed
description BACKGROUND: Hepatitis B virus (HBV) infection and its sequelae are now recognized as serious problems globally. Our aime is to screen hepatocellular carcinoma (HCC) from chronic hepatitis B (CHB) and identify the characteristics of proteins involved. METHODOLOGY/PRINCIPAL FINDINGS: We affinity-purified sample serum with weak cation-exchange (WCX) magnetic beads and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis to search for potential markers. The 4210 Da protein, which differed substantially between HCC and CHB isolates, was later identified to be eukaryotic peptide chain release factor GTP-binding subunit eRF3b. Further research showed that eRF3b/GSPT2 was positively expressed in liver tissues. GSPT2 mRNA was, however differentially expressed in blood. Compared with normal controls, the relative expression of GSPT2/18s rRNA was higher in CHB patients than in patients with either LC or HCC (P = 0.035 for CHB vs. LC; P = 0.020 for CHB vs. HCC). The data of further research showed that eRF3b/GSPT2 promoted the entrance of the HepG2 cells into the S-phase and that one of the substrates of the mTOR kinase, 4E-BP1, was hyperphosphorylated in eRF3b-overexpressing HepG2 cells. CONCLUSIONS: Overall, the differentially expressed protein eRF3b, which was discovered as a biomarker for HCC, could change the cell cycle and influence the phosphorylation status of 4E-BP1 on Ser65 in HepG2.
format Online
Article
Text
id pubmed-3900531
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39005312014-01-24 eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1 Li, Man Wang, Jian Yang, Lei Gao, Ping Tian, Qing-bao Liu, Dian-wu PLoS One Research Article BACKGROUND: Hepatitis B virus (HBV) infection and its sequelae are now recognized as serious problems globally. Our aime is to screen hepatocellular carcinoma (HCC) from chronic hepatitis B (CHB) and identify the characteristics of proteins involved. METHODOLOGY/PRINCIPAL FINDINGS: We affinity-purified sample serum with weak cation-exchange (WCX) magnetic beads and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis to search for potential markers. The 4210 Da protein, which differed substantially between HCC and CHB isolates, was later identified to be eukaryotic peptide chain release factor GTP-binding subunit eRF3b. Further research showed that eRF3b/GSPT2 was positively expressed in liver tissues. GSPT2 mRNA was, however differentially expressed in blood. Compared with normal controls, the relative expression of GSPT2/18s rRNA was higher in CHB patients than in patients with either LC or HCC (P = 0.035 for CHB vs. LC; P = 0.020 for CHB vs. HCC). The data of further research showed that eRF3b/GSPT2 promoted the entrance of the HepG2 cells into the S-phase and that one of the substrates of the mTOR kinase, 4E-BP1, was hyperphosphorylated in eRF3b-overexpressing HepG2 cells. CONCLUSIONS: Overall, the differentially expressed protein eRF3b, which was discovered as a biomarker for HCC, could change the cell cycle and influence the phosphorylation status of 4E-BP1 on Ser65 in HepG2. Public Library of Science 2014-01-23 /pmc/articles/PMC3900531/ /pubmed/24466059 http://dx.doi.org/10.1371/journal.pone.0086371 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Man
Wang, Jian
Yang, Lei
Gao, Ping
Tian, Qing-bao
Liu, Dian-wu
eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title_full eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title_fullStr eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title_full_unstemmed eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title_short eRF3b, a Biomarker for Hepatocellular Carcinoma, Influences Cell Cycle and Phosphoralation Status of 4E-BP1
title_sort erf3b, a biomarker for hepatocellular carcinoma, influences cell cycle and phosphoralation status of 4e-bp1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900531/
https://www.ncbi.nlm.nih.gov/pubmed/24466059
http://dx.doi.org/10.1371/journal.pone.0086371
work_keys_str_mv AT liman erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1
AT wangjian erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1
AT yanglei erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1
AT gaoping erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1
AT tianqingbao erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1
AT liudianwu erf3babiomarkerforhepatocellularcarcinomainfluencescellcycleandphosphoralationstatusof4ebp1