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Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa

BACKGROUND: Inferring of parentage in natural populations is important in understanding the mating systems of a species, which have great effects on its genetic structure and evolution. Muricidae, a large group (approximately 1,600 species) of marine gastropods, are poorly investigated in patterns o...

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Autores principales: Xue, Dongxiu, Zhang, Tao, Liu, Jin-Xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900555/
https://www.ncbi.nlm.nih.gov/pubmed/24466127
http://dx.doi.org/10.1371/journal.pone.0086508
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author Xue, Dongxiu
Zhang, Tao
Liu, Jin-Xian
author_facet Xue, Dongxiu
Zhang, Tao
Liu, Jin-Xian
author_sort Xue, Dongxiu
collection PubMed
description BACKGROUND: Inferring of parentage in natural populations is important in understanding the mating systems of a species, which have great effects on its genetic structure and evolution. Muricidae, a large group (approximately 1,600 species) of marine gastropods, are poorly investigated in patterns of multiple paternity and sperm competition based on molecular techniques. The veined Rapa whelk, Rapana venosa, a commercially important muricid species with internal fertilization, is an ideal species to study the occurrence and frequency of multiple paternity and to facilitate understanding of their reproductive strategies. METHODOLOGY/PRINCIPAL FINDINGS: We developed five highly polymorphic microsatellites in R. venosa and applied them to identify multiple paternity in 19 broods (1381 embryos) collected from Dandong, China. Multiple paternity was detected in 17 (89.5%) of 19 broods. The number of sires per brood ranged from 1 to 7 (4.3 on average). Of the 17 multiply sired broods, 16 (94.1%) were significantly skewed from equal paternal contributions, and had a dominant sire which was also dominant in each assayed capsule. CONCLUSIONS: Our results indicate that a high level of multiple paternity occurs in the wild population of R. venosa. Similar patterns of multiple paternity in the 2–6 assayed capsules from each brood imply that fertilization events within the body of a female occur mostly (but not entirely) as random draws from a “well-but-not-perfectly blended sperm pool” of her several mates. Strongly skewed distributions of fertilization success among sires also suggest that sperm competition and/or cryptic female choice might be important for post-copulatory paternity biasing in this species.
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spelling pubmed-39005552014-01-24 Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa Xue, Dongxiu Zhang, Tao Liu, Jin-Xian PLoS One Research Article BACKGROUND: Inferring of parentage in natural populations is important in understanding the mating systems of a species, which have great effects on its genetic structure and evolution. Muricidae, a large group (approximately 1,600 species) of marine gastropods, are poorly investigated in patterns of multiple paternity and sperm competition based on molecular techniques. The veined Rapa whelk, Rapana venosa, a commercially important muricid species with internal fertilization, is an ideal species to study the occurrence and frequency of multiple paternity and to facilitate understanding of their reproductive strategies. METHODOLOGY/PRINCIPAL FINDINGS: We developed five highly polymorphic microsatellites in R. venosa and applied them to identify multiple paternity in 19 broods (1381 embryos) collected from Dandong, China. Multiple paternity was detected in 17 (89.5%) of 19 broods. The number of sires per brood ranged from 1 to 7 (4.3 on average). Of the 17 multiply sired broods, 16 (94.1%) were significantly skewed from equal paternal contributions, and had a dominant sire which was also dominant in each assayed capsule. CONCLUSIONS: Our results indicate that a high level of multiple paternity occurs in the wild population of R. venosa. Similar patterns of multiple paternity in the 2–6 assayed capsules from each brood imply that fertilization events within the body of a female occur mostly (but not entirely) as random draws from a “well-but-not-perfectly blended sperm pool” of her several mates. Strongly skewed distributions of fertilization success among sires also suggest that sperm competition and/or cryptic female choice might be important for post-copulatory paternity biasing in this species. Public Library of Science 2014-01-23 /pmc/articles/PMC3900555/ /pubmed/24466127 http://dx.doi.org/10.1371/journal.pone.0086508 Text en © 2014 Xue et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xue, Dongxiu
Zhang, Tao
Liu, Jin-Xian
Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title_full Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title_fullStr Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title_full_unstemmed Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title_short Microsatellite Evidence for High Frequency of Multiple Paternity in the Marine Gastropod Rapana venosa
title_sort microsatellite evidence for high frequency of multiple paternity in the marine gastropod rapana venosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900555/
https://www.ncbi.nlm.nih.gov/pubmed/24466127
http://dx.doi.org/10.1371/journal.pone.0086508
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