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Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy

We have demonstrated that gold nanocage-photosensitizer conjugates can enable dual image-guided delivery of photosensitizer and significantly improve the efficacy of photodynamic therapy in a murine model. The photosensitizer, 3-devinyl-3-(1'-hexyloxyethyl)pyropheophorbide (HPPH), was noncovale...

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Autores principales: Srivatsan, Avinash, Jenkins, Samir V., Jeon, Mansik, Wu, Zhijin, Kim, Chulhong, Chen, Jingyi, Pandey, Ravindra K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900801/
https://www.ncbi.nlm.nih.gov/pubmed/24465274
http://dx.doi.org/10.7150/thno.7064
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author Srivatsan, Avinash
Jenkins, Samir V.
Jeon, Mansik
Wu, Zhijin
Kim, Chulhong
Chen, Jingyi
Pandey, Ravindra K.
author_facet Srivatsan, Avinash
Jenkins, Samir V.
Jeon, Mansik
Wu, Zhijin
Kim, Chulhong
Chen, Jingyi
Pandey, Ravindra K.
author_sort Srivatsan, Avinash
collection PubMed
description We have demonstrated that gold nanocage-photosensitizer conjugates can enable dual image-guided delivery of photosensitizer and significantly improve the efficacy of photodynamic therapy in a murine model. The photosensitizer, 3-devinyl-3-(1'-hexyloxyethyl)pyropheophorbide (HPPH), was noncovalently entrapped in the poly(ethylene glycol) monolayer coated on the surface of gold nanocages. The conjugate is stable in saline solutions, while incubation in protein rich solutions leads to gradual unloading of the HPPH, which can be monitored optically by fluorescence and photoacoustic imaging. The slow nature of the release in turn results in an increase in accumulation of the drug within implanted tumors due to the passive delivery of gold nanocages. Furthermore, the conjugate is found to generate more therapeutic singlet oxygen and have a lower IC(50) value than the free drug alone. Thus the conjugate shows significant suppression of tumor growth as compared to the free drug in vivo. Short-term study showed neither toxicity nor phenotypical changes in mice at therapeutic dose of the conjugates or even at 100-fold higher than therapeutic dose of gold nanocages.
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spelling pubmed-39008012014-01-24 Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy Srivatsan, Avinash Jenkins, Samir V. Jeon, Mansik Wu, Zhijin Kim, Chulhong Chen, Jingyi Pandey, Ravindra K. Theranostics Research Paper We have demonstrated that gold nanocage-photosensitizer conjugates can enable dual image-guided delivery of photosensitizer and significantly improve the efficacy of photodynamic therapy in a murine model. The photosensitizer, 3-devinyl-3-(1'-hexyloxyethyl)pyropheophorbide (HPPH), was noncovalently entrapped in the poly(ethylene glycol) monolayer coated on the surface of gold nanocages. The conjugate is stable in saline solutions, while incubation in protein rich solutions leads to gradual unloading of the HPPH, which can be monitored optically by fluorescence and photoacoustic imaging. The slow nature of the release in turn results in an increase in accumulation of the drug within implanted tumors due to the passive delivery of gold nanocages. Furthermore, the conjugate is found to generate more therapeutic singlet oxygen and have a lower IC(50) value than the free drug alone. Thus the conjugate shows significant suppression of tumor growth as compared to the free drug in vivo. Short-term study showed neither toxicity nor phenotypical changes in mice at therapeutic dose of the conjugates or even at 100-fold higher than therapeutic dose of gold nanocages. Ivyspring International Publisher 2014-01-05 /pmc/articles/PMC3900801/ /pubmed/24465274 http://dx.doi.org/10.7150/thno.7064 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Srivatsan, Avinash
Jenkins, Samir V.
Jeon, Mansik
Wu, Zhijin
Kim, Chulhong
Chen, Jingyi
Pandey, Ravindra K.
Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title_full Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title_fullStr Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title_full_unstemmed Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title_short Gold Nanocage-Photosensitizer Conjugates for Dual-Modal Image-Guided Enhanced Photodynamic Therapy
title_sort gold nanocage-photosensitizer conjugates for dual-modal image-guided enhanced photodynamic therapy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900801/
https://www.ncbi.nlm.nih.gov/pubmed/24465274
http://dx.doi.org/10.7150/thno.7064
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