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The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation

The quality of super resolution images obtained by stochastic single-molecule microscopy critically depends on image analysis algorithms. We find that the choice of background estimator is often the most important determinant of reconstruction quality. A variety of techniques have found use, but man...

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Detalles Bibliográficos
Autores principales: Hoogendoorn, Eelco, Crosby, Kevin C., Leyton-Puig, Daniela, Breedijk, Ronald M. P., Jalink, Kees, Gadella, Theodorus W. J., Postma, Marten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900998/
https://www.ncbi.nlm.nih.gov/pubmed/24458236
http://dx.doi.org/10.1038/srep03854