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New aspects of Saccharomyces cerevisiae as a novel carrier for berberine
BACKGROUND: Berberine was encapsulated in yeast cells of Saccharomyces cerevisiae as novel carriers to be used in different food and drug industries. The microcapsules were characterized by differential scanning calorimetry (DSC), fourier transform infra red spectroscopy (FT-IR) and fluorescence mic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901020/ https://www.ncbi.nlm.nih.gov/pubmed/24359687 http://dx.doi.org/10.1186/2008-2231-21-73 |
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author | Salari, Roshanak Bazzaz, BiBi Sedigheh Fazly Rajabi, Omid Khashyarmanesh, Zahra |
author_facet | Salari, Roshanak Bazzaz, BiBi Sedigheh Fazly Rajabi, Omid Khashyarmanesh, Zahra |
author_sort | Salari, Roshanak |
collection | PubMed |
description | BACKGROUND: Berberine was encapsulated in yeast cells of Saccharomyces cerevisiae as novel carriers to be used in different food and drug industries. The microcapsules were characterized by differential scanning calorimetry (DSC), fourier transform infra red spectroscopy (FT-IR) and fluorescence microscopy. The encapsulation factors such as plasmolysis of yeast cells which affects the % encapsulation yield were studied. RESULTS: Fluorescence microscopy showed the yeast cells became fluorescent after encapsulation process. DSC diagram was representing of new peak for microcapsule which was not the same as berberine and the empty yeast cells peaks, separately. FTIR spectrums of microcapsules and yeast cells were almost the same. The plasmolysed and non plasmolysed microcapsules were loaded with berberine up to about 40.2 ± 0.2% w/w. CONCLUSION: Analytical methods proved that berberine was encapsulated in the yeast cells. Fluorescence microscopy and FTIR results showed the entrance of berberine inside the yeasts. DSC diagram indicated the appearance of new peak which is due to the synthesis of new product. Although plasmolysis caused changes in yeast cell structure and properties, it did not enhance berberine loading in the cells. The results confirmed that Saccharomyces cerevisiae could be an efficient and safe carrier for active materials. |
format | Online Article Text |
id | pubmed-3901020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39010202014-02-06 New aspects of Saccharomyces cerevisiae as a novel carrier for berberine Salari, Roshanak Bazzaz, BiBi Sedigheh Fazly Rajabi, Omid Khashyarmanesh, Zahra Daru Research Article BACKGROUND: Berberine was encapsulated in yeast cells of Saccharomyces cerevisiae as novel carriers to be used in different food and drug industries. The microcapsules were characterized by differential scanning calorimetry (DSC), fourier transform infra red spectroscopy (FT-IR) and fluorescence microscopy. The encapsulation factors such as plasmolysis of yeast cells which affects the % encapsulation yield were studied. RESULTS: Fluorescence microscopy showed the yeast cells became fluorescent after encapsulation process. DSC diagram was representing of new peak for microcapsule which was not the same as berberine and the empty yeast cells peaks, separately. FTIR spectrums of microcapsules and yeast cells were almost the same. The plasmolysed and non plasmolysed microcapsules were loaded with berberine up to about 40.2 ± 0.2% w/w. CONCLUSION: Analytical methods proved that berberine was encapsulated in the yeast cells. Fluorescence microscopy and FTIR results showed the entrance of berberine inside the yeasts. DSC diagram indicated the appearance of new peak which is due to the synthesis of new product. Although plasmolysis caused changes in yeast cell structure and properties, it did not enhance berberine loading in the cells. The results confirmed that Saccharomyces cerevisiae could be an efficient and safe carrier for active materials. BioMed Central 2013-12-20 /pmc/articles/PMC3901020/ /pubmed/24359687 http://dx.doi.org/10.1186/2008-2231-21-73 Text en Copyright © 2013 Salari et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Salari, Roshanak Bazzaz, BiBi Sedigheh Fazly Rajabi, Omid Khashyarmanesh, Zahra New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title | New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title_full | New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title_fullStr | New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title_full_unstemmed | New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title_short | New aspects of Saccharomyces cerevisiae as a novel carrier for berberine |
title_sort | new aspects of saccharomyces cerevisiae as a novel carrier for berberine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901020/ https://www.ncbi.nlm.nih.gov/pubmed/24359687 http://dx.doi.org/10.1186/2008-2231-21-73 |
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