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Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural dece...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901141/ https://www.ncbi.nlm.nih.gov/pubmed/24710540 http://dx.doi.org/10.3390/cells1041010 |
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author | Schulze-Tanzil, Gundula Al-Sadi, Onays Ertel, Wolfgang Lohan, Anke |
author_facet | Schulze-Tanzil, Gundula Al-Sadi, Onays Ertel, Wolfgang Lohan, Anke |
author_sort | Schulze-Tanzil, Gundula |
collection | PubMed |
description | Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural decellularized allo- or xenogenic tendon extracellular matrix (ECM) have recently started to attract interest. This ECM combines the advantages of its intrinsic mechanical competence with that of providing tenogenic stimuli for immigrating cells mediated, for example, by the growth factors and other mediators entrapped within the natural ECM. A major restriction for their therapeutic application is the mainly cell-associated immunogenicity of xenogenic or allogenic tissues and, in the case of allogenic tissues, also the risk of disease transmission. A survey of approaches for tendon reconstruction using cell-free tendon ECM is presented here, whereby the problems associated with the decellularization procedures, the success of various recellularization strategies, and the applicable cell types will be thoroughly discussed. Encouraging in vivo results using cell-free ECM, as, for instance, in rabbit models, have already been reported. However, in comparison to native tendon, cells remain mostly inhomogeneously distributed in the reseeded ECM and do not align. Hence, future work should focus on the optimization of tendon ECM decellularization and recolonization strategies to restore tendon functionality. |
format | Online Article Text |
id | pubmed-3901141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-39011412014-04-07 Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? Schulze-Tanzil, Gundula Al-Sadi, Onays Ertel, Wolfgang Lohan, Anke Cells Review Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural decellularized allo- or xenogenic tendon extracellular matrix (ECM) have recently started to attract interest. This ECM combines the advantages of its intrinsic mechanical competence with that of providing tenogenic stimuli for immigrating cells mediated, for example, by the growth factors and other mediators entrapped within the natural ECM. A major restriction for their therapeutic application is the mainly cell-associated immunogenicity of xenogenic or allogenic tissues and, in the case of allogenic tissues, also the risk of disease transmission. A survey of approaches for tendon reconstruction using cell-free tendon ECM is presented here, whereby the problems associated with the decellularization procedures, the success of various recellularization strategies, and the applicable cell types will be thoroughly discussed. Encouraging in vivo results using cell-free ECM, as, for instance, in rabbit models, have already been reported. However, in comparison to native tendon, cells remain mostly inhomogeneously distributed in the reseeded ECM and do not align. Hence, future work should focus on the optimization of tendon ECM decellularization and recolonization strategies to restore tendon functionality. MDPI 2012-11-05 /pmc/articles/PMC3901141/ /pubmed/24710540 http://dx.doi.org/10.3390/cells1041010 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Schulze-Tanzil, Gundula Al-Sadi, Onays Ertel, Wolfgang Lohan, Anke Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title | Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title_full | Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title_fullStr | Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title_full_unstemmed | Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title_short | Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? |
title_sort | decellularized tendon extracellular matrix—a valuable approach for tendon reconstruction? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901141/ https://www.ncbi.nlm.nih.gov/pubmed/24710540 http://dx.doi.org/10.3390/cells1041010 |
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