Cargando…

Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?

Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural dece...

Descripción completa

Detalles Bibliográficos
Autores principales: Schulze-Tanzil, Gundula, Al-Sadi, Onays, Ertel, Wolfgang, Lohan, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901141/
https://www.ncbi.nlm.nih.gov/pubmed/24710540
http://dx.doi.org/10.3390/cells1041010
_version_ 1782300806955925504
author Schulze-Tanzil, Gundula
Al-Sadi, Onays
Ertel, Wolfgang
Lohan, Anke
author_facet Schulze-Tanzil, Gundula
Al-Sadi, Onays
Ertel, Wolfgang
Lohan, Anke
author_sort Schulze-Tanzil, Gundula
collection PubMed
description Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural decellularized allo- or xenogenic tendon extracellular matrix (ECM) have recently started to attract interest. This ECM combines the advantages of its intrinsic mechanical competence with that of providing tenogenic stimuli for immigrating cells mediated, for example, by the growth factors and other mediators entrapped within the natural ECM. A major restriction for their therapeutic application is the mainly cell-associated immunogenicity of xenogenic or allogenic tissues and, in the case of allogenic tissues, also the risk of disease transmission. A survey of approaches for tendon reconstruction using cell-free tendon ECM is presented here, whereby the problems associated with the decellularization procedures, the success of various recellularization strategies, and the applicable cell types will be thoroughly discussed. Encouraging in vivo results using cell-free ECM, as, for instance, in rabbit models, have already been reported. However, in comparison to native tendon, cells remain mostly inhomogeneously distributed in the reseeded ECM and do not align. Hence, future work should focus on the optimization of tendon ECM decellularization and recolonization strategies to restore tendon functionality.
format Online
Article
Text
id pubmed-3901141
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-39011412014-04-07 Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction? Schulze-Tanzil, Gundula Al-Sadi, Onays Ertel, Wolfgang Lohan, Anke Cells Review Tendon healing is generally a time-consuming process and often leads to a functionally altered reparative tissue. Using degradable scaffolds for tendon reconstruction still remains a compromise in view of the required high mechanical strength of tendons. Regenerative approaches based on natural decellularized allo- or xenogenic tendon extracellular matrix (ECM) have recently started to attract interest. This ECM combines the advantages of its intrinsic mechanical competence with that of providing tenogenic stimuli for immigrating cells mediated, for example, by the growth factors and other mediators entrapped within the natural ECM. A major restriction for their therapeutic application is the mainly cell-associated immunogenicity of xenogenic or allogenic tissues and, in the case of allogenic tissues, also the risk of disease transmission. A survey of approaches for tendon reconstruction using cell-free tendon ECM is presented here, whereby the problems associated with the decellularization procedures, the success of various recellularization strategies, and the applicable cell types will be thoroughly discussed. Encouraging in vivo results using cell-free ECM, as, for instance, in rabbit models, have already been reported. However, in comparison to native tendon, cells remain mostly inhomogeneously distributed in the reseeded ECM and do not align. Hence, future work should focus on the optimization of tendon ECM decellularization and recolonization strategies to restore tendon functionality. MDPI 2012-11-05 /pmc/articles/PMC3901141/ /pubmed/24710540 http://dx.doi.org/10.3390/cells1041010 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Schulze-Tanzil, Gundula
Al-Sadi, Onays
Ertel, Wolfgang
Lohan, Anke
Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title_full Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title_fullStr Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title_full_unstemmed Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title_short Decellularized Tendon Extracellular Matrix—A Valuable Approach for Tendon Reconstruction?
title_sort decellularized tendon extracellular matrix—a valuable approach for tendon reconstruction?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901141/
https://www.ncbi.nlm.nih.gov/pubmed/24710540
http://dx.doi.org/10.3390/cells1041010
work_keys_str_mv AT schulzetanzilgundula decellularizedtendonextracellularmatrixavaluableapproachfortendonreconstruction
AT alsadionays decellularizedtendonextracellularmatrixavaluableapproachfortendonreconstruction
AT ertelwolfgang decellularizedtendonextracellularmatrixavaluableapproachfortendonreconstruction
AT lohananke decellularizedtendonextracellularmatrixavaluableapproachfortendonreconstruction