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Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples

Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be diffic...

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Detalles Bibliográficos
Autores principales: Iwasaki, Yusuke, Sawada, Takahiro, Hatayama, Kentaro, Ohyagi, Akihito, Tsukuda, Yuri, Namekawa, Kyohei, Ito, Rie, Saito, Koichi, Nakazawa, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901216/
https://www.ncbi.nlm.nih.gov/pubmed/24957644
http://dx.doi.org/10.3390/metabo2030496
Descripción
Sumario:Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be difficult to separate highly polar compounds. Mass spectrometry (MS), in combination with capillary electrophoresis (CE), gas chromatography (GC), or high performance liquid chromatography (HPLC) is the key analytical technique on which emerging "omics" technologies, namely, proteomics, metabolomics, and lipidomics, are based. In this review, we introduce various methods for the separation of highly polar metabolites.