Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples

Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be diffic...

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Detalles Bibliográficos
Autores principales: Iwasaki, Yusuke, Sawada, Takahiro, Hatayama, Kentaro, Ohyagi, Akihito, Tsukuda, Yuri, Namekawa, Kyohei, Ito, Rie, Saito, Koichi, Nakazawa, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901216/
https://www.ncbi.nlm.nih.gov/pubmed/24957644
http://dx.doi.org/10.3390/metabo2030496
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author Iwasaki, Yusuke
Sawada, Takahiro
Hatayama, Kentaro
Ohyagi, Akihito
Tsukuda, Yuri
Namekawa, Kyohei
Ito, Rie
Saito, Koichi
Nakazawa, Hiroyuki
author_facet Iwasaki, Yusuke
Sawada, Takahiro
Hatayama, Kentaro
Ohyagi, Akihito
Tsukuda, Yuri
Namekawa, Kyohei
Ito, Rie
Saito, Koichi
Nakazawa, Hiroyuki
author_sort Iwasaki, Yusuke
collection PubMed
description Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be difficult to separate highly polar compounds. Mass spectrometry (MS), in combination with capillary electrophoresis (CE), gas chromatography (GC), or high performance liquid chromatography (HPLC) is the key analytical technique on which emerging "omics" technologies, namely, proteomics, metabolomics, and lipidomics, are based. In this review, we introduce various methods for the separation of highly polar metabolites.
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spelling pubmed-39012162014-05-27 Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples Iwasaki, Yusuke Sawada, Takahiro Hatayama, Kentaro Ohyagi, Akihito Tsukuda, Yuri Namekawa, Kyohei Ito, Rie Saito, Koichi Nakazawa, Hiroyuki Metabolites Review Metabolomics is a new approach that is based on the systematic study of the full complement of metabolites in a biological sample. Metabolomics has the potential to fundamentally change clinical chemistry and, by extension, the fields of nutrition, toxicology, and medicine. However, it can be difficult to separate highly polar compounds. Mass spectrometry (MS), in combination with capillary electrophoresis (CE), gas chromatography (GC), or high performance liquid chromatography (HPLC) is the key analytical technique on which emerging "omics" technologies, namely, proteomics, metabolomics, and lipidomics, are based. In this review, we introduce various methods for the separation of highly polar metabolites. MDPI 2012-08-16 /pmc/articles/PMC3901216/ /pubmed/24957644 http://dx.doi.org/10.3390/metabo2030496 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Iwasaki, Yusuke
Sawada, Takahiro
Hatayama, Kentaro
Ohyagi, Akihito
Tsukuda, Yuri
Namekawa, Kyohei
Ito, Rie
Saito, Koichi
Nakazawa, Hiroyuki
Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title_full Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title_fullStr Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title_full_unstemmed Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title_short Separation Technique for the Determination of Highly Polar Metabolites in Biological Samples
title_sort separation technique for the determination of highly polar metabolites in biological samples
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901216/
https://www.ncbi.nlm.nih.gov/pubmed/24957644
http://dx.doi.org/10.3390/metabo2030496
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