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(1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats

(1)H NMR spectroscopy and chemometric analysis were used to characterize rat urine obtained after chronic exposure to either tributyl phosphate (TBP) or triphenyl phosphate (TPP). In this study, the daily dose exposure was 1.5 mg/kg body weight for TBP, or 2.0 mg/kg body weight for TPP, administered...

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Autores principales: Alam, Todd M., Neerathilingam, Muniasamy, Alam, M. Kathleen, Volk, David E., Ansari, G. A. Shakeel, Sarkar, Swapna, Luxon, Bruce A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901221/
https://www.ncbi.nlm.nih.gov/pubmed/24957643
http://dx.doi.org/10.3390/metabo2030479
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author Alam, Todd M.
Neerathilingam, Muniasamy
Alam, M. Kathleen
Volk, David E.
Ansari, G. A. Shakeel
Sarkar, Swapna
Luxon, Bruce A.
author_facet Alam, Todd M.
Neerathilingam, Muniasamy
Alam, M. Kathleen
Volk, David E.
Ansari, G. A. Shakeel
Sarkar, Swapna
Luxon, Bruce A.
author_sort Alam, Todd M.
collection PubMed
description (1)H NMR spectroscopy and chemometric analysis were used to characterize rat urine obtained after chronic exposure to either tributyl phosphate (TBP) or triphenyl phosphate (TPP). In this study, the daily dose exposure was 1.5 mg/kg body weight for TBP, or 2.0 mg/kg body weight for TPP, administered over a 15-week period. Orthogonal signal correction (OSC) -filtered partial least square discriminant analysis (OSC-PLSDA) was used to predict and classify exposure to these organophosphates. During the development of the model, the classification error was evaluated as a function of the number of latent variables. NMR spectral regions and corresponding metabolites important for determination of exposure type were identified using variable importance in projection (VIP) coefficients obtained from the OSC-PLSDA analysis. As expected, the model for classification of chronic (1.5–2.0 mg/kg body weight daily) TBP or TPP exposure was not as strong as the previously reported model developed for identifying acute (15–20 mg/kg body weight) exposure. The set of majorly impacted metabolites identified for chronic TBP or TPP exposure was slightly different than those metabolites previously identified for acute exposure. These metabolites were then mapped to different metabolite pathways and ranked, allowing the metabolic response to chronic organophosphate exposure to be addressed.
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spelling pubmed-39012212014-05-27 (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats Alam, Todd M. Neerathilingam, Muniasamy Alam, M. Kathleen Volk, David E. Ansari, G. A. Shakeel Sarkar, Swapna Luxon, Bruce A. Metabolites Article (1)H NMR spectroscopy and chemometric analysis were used to characterize rat urine obtained after chronic exposure to either tributyl phosphate (TBP) or triphenyl phosphate (TPP). In this study, the daily dose exposure was 1.5 mg/kg body weight for TBP, or 2.0 mg/kg body weight for TPP, administered over a 15-week period. Orthogonal signal correction (OSC) -filtered partial least square discriminant analysis (OSC-PLSDA) was used to predict and classify exposure to these organophosphates. During the development of the model, the classification error was evaluated as a function of the number of latent variables. NMR spectral regions and corresponding metabolites important for determination of exposure type were identified using variable importance in projection (VIP) coefficients obtained from the OSC-PLSDA analysis. As expected, the model for classification of chronic (1.5–2.0 mg/kg body weight daily) TBP or TPP exposure was not as strong as the previously reported model developed for identifying acute (15–20 mg/kg body weight) exposure. The set of majorly impacted metabolites identified for chronic TBP or TPP exposure was slightly different than those metabolites previously identified for acute exposure. These metabolites were then mapped to different metabolite pathways and ranked, allowing the metabolic response to chronic organophosphate exposure to be addressed. MDPI 2012-07-24 /pmc/articles/PMC3901221/ /pubmed/24957643 http://dx.doi.org/10.3390/metabo2030479 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Alam, Todd M.
Neerathilingam, Muniasamy
Alam, M. Kathleen
Volk, David E.
Ansari, G. A. Shakeel
Sarkar, Swapna
Luxon, Bruce A.
(1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title_full (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title_fullStr (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title_full_unstemmed (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title_short (1)H Nuclear Magnetic Resonance (NMR) Metabolomic Study of Chronic Organophosphate Exposure in Rats
title_sort (1)h nuclear magnetic resonance (nmr) metabolomic study of chronic organophosphate exposure in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901221/
https://www.ncbi.nlm.nih.gov/pubmed/24957643
http://dx.doi.org/10.3390/metabo2030479
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