Chemical Synthesis of U1 snRNA Derivatives

[Image: see text] U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the b...

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Detalles Bibliográficos
Autores principales: Ohkubo, Akihiro, Kondo, Yasushi, Suzuki, Makoto, Kobayashi, Haruki, Kanamori, Takashi, Masaki, Yoshiaki, Seio, Kohji, Nagai, Kiyoshi, Sekine, Mitsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901379/
https://www.ncbi.nlm.nih.gov/pubmed/23952175
http://dx.doi.org/10.1021/ol401917r
Descripción
Sumario:[Image: see text] U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA.

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