Chemical Synthesis of U1 snRNA Derivatives

[Image: see text] U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the b...

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Autores principales: Ohkubo, Akihiro, Kondo, Yasushi, Suzuki, Makoto, Kobayashi, Haruki, Kanamori, Takashi, Masaki, Yoshiaki, Seio, Kohji, Nagai, Kiyoshi, Sekine, Mitsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901379/
https://www.ncbi.nlm.nih.gov/pubmed/23952175
http://dx.doi.org/10.1021/ol401917r
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author Ohkubo, Akihiro
Kondo, Yasushi
Suzuki, Makoto
Kobayashi, Haruki
Kanamori, Takashi
Masaki, Yoshiaki
Seio, Kohji
Nagai, Kiyoshi
Sekine, Mitsuo
author_facet Ohkubo, Akihiro
Kondo, Yasushi
Suzuki, Makoto
Kobayashi, Haruki
Kanamori, Takashi
Masaki, Yoshiaki
Seio, Kohji
Nagai, Kiyoshi
Sekine, Mitsuo
author_sort Ohkubo, Akihiro
collection PubMed
description [Image: see text] U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA.
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spelling pubmed-39013792014-01-24 Chemical Synthesis of U1 snRNA Derivatives Ohkubo, Akihiro Kondo, Yasushi Suzuki, Makoto Kobayashi, Haruki Kanamori, Takashi Masaki, Yoshiaki Seio, Kohji Nagai, Kiyoshi Sekine, Mitsuo Org Lett [Image: see text] U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA. American Chemical Society 2013-08-16 2013-09-06 /pmc/articles/PMC3901379/ /pubmed/23952175 http://dx.doi.org/10.1021/ol401917r Text en Copyright © 2013 American Chemical Society Terms of Use CC-BY-NC-ND (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html)
spellingShingle Ohkubo, Akihiro
Kondo, Yasushi
Suzuki, Makoto
Kobayashi, Haruki
Kanamori, Takashi
Masaki, Yoshiaki
Seio, Kohji
Nagai, Kiyoshi
Sekine, Mitsuo
Chemical Synthesis of U1 snRNA Derivatives
title Chemical Synthesis of U1 snRNA Derivatives
title_full Chemical Synthesis of U1 snRNA Derivatives
title_fullStr Chemical Synthesis of U1 snRNA Derivatives
title_full_unstemmed Chemical Synthesis of U1 snRNA Derivatives
title_short Chemical Synthesis of U1 snRNA Derivatives
title_sort chemical synthesis of u1 snrna derivatives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901379/
https://www.ncbi.nlm.nih.gov/pubmed/23952175
http://dx.doi.org/10.1021/ol401917r
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