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SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric canc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902720/ https://www.ncbi.nlm.nih.gov/pubmed/24482641 http://dx.doi.org/10.5114/aoms.2013.39790 |
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author | Zhao, Kui Zhu, Bao-Song Gong, Wei Zhu, Mo-Li Gao, Zhi-Tao Wu, Yong-You Chen, Qiang Yang, Xiao-Dong Xing, Chun-Gen |
author_facet | Zhao, Kui Zhu, Bao-Song Gong, Wei Zhu, Mo-Li Gao, Zhi-Tao Wu, Yong-You Chen, Qiang Yang, Xiao-Dong Xing, Chun-Gen |
author_sort | Zhao, Kui |
collection | PubMed |
description | INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric cancer of LY294002 in vitro, and we also wanted to know the mechanism of it, which might be applied to clinical tumor therapy. MATERIAL AND METHODS: The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effects of the drugs. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Hoechst 33258 staining was used to detect apoptosis and necrosis morphological changes after LY294002 and/or SN50 treatment. Expression of p53, PUMA and Beclin1 were determined with real-time polymerase chain reaction (RT-PCR) analysis. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after LY294002 and/or SN50 treatment. RESULTS: In this study, we found that treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h, compared to the control group (p < 0.05). Detection of mitochondrial potential and transmission electron microscopic examination indicated that the rate of cell death increased progressively. The expression of p53, PUMA and Beclin1 was up-regulated. CONCLUSIONS: The NF-κB inhibitor SN50 could enhance the role of LY294002 on inducing cell death of human gastric cancer cells SGC7901, which might be a promising new approach to gastric cancer therapy. |
format | Online Article Text |
id | pubmed-3902720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-39027202014-01-30 SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 Zhao, Kui Zhu, Bao-Song Gong, Wei Zhu, Mo-Li Gao, Zhi-Tao Wu, Yong-You Chen, Qiang Yang, Xiao-Dong Xing, Chun-Gen Arch Med Sci Basic Research INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric cancer of LY294002 in vitro, and we also wanted to know the mechanism of it, which might be applied to clinical tumor therapy. MATERIAL AND METHODS: The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effects of the drugs. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Hoechst 33258 staining was used to detect apoptosis and necrosis morphological changes after LY294002 and/or SN50 treatment. Expression of p53, PUMA and Beclin1 were determined with real-time polymerase chain reaction (RT-PCR) analysis. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after LY294002 and/or SN50 treatment. RESULTS: In this study, we found that treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h, compared to the control group (p < 0.05). Detection of mitochondrial potential and transmission electron microscopic examination indicated that the rate of cell death increased progressively. The expression of p53, PUMA and Beclin1 was up-regulated. CONCLUSIONS: The NF-κB inhibitor SN50 could enhance the role of LY294002 on inducing cell death of human gastric cancer cells SGC7901, which might be a promising new approach to gastric cancer therapy. Termedia Publishing House 2013-12-26 2013-12-30 /pmc/articles/PMC3902720/ /pubmed/24482641 http://dx.doi.org/10.5114/aoms.2013.39790 Text en Copyright © 2013 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Basic Research Zhao, Kui Zhu, Bao-Song Gong, Wei Zhu, Mo-Li Gao, Zhi-Tao Wu, Yong-You Chen, Qiang Yang, Xiao-Dong Xing, Chun-Gen SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title | SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title_full | SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title_fullStr | SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title_full_unstemmed | SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title_short | SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 |
title_sort | sn50 enhances the effects of ly294002 on cell death induction in gastric cancer cell line sgc7901 |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902720/ https://www.ncbi.nlm.nih.gov/pubmed/24482641 http://dx.doi.org/10.5114/aoms.2013.39790 |
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