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SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901

INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric canc...

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Autores principales: Zhao, Kui, Zhu, Bao-Song, Gong, Wei, Zhu, Mo-Li, Gao, Zhi-Tao, Wu, Yong-You, Chen, Qiang, Yang, Xiao-Dong, Xing, Chun-Gen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902720/
https://www.ncbi.nlm.nih.gov/pubmed/24482641
http://dx.doi.org/10.5114/aoms.2013.39790
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author Zhao, Kui
Zhu, Bao-Song
Gong, Wei
Zhu, Mo-Li
Gao, Zhi-Tao
Wu, Yong-You
Chen, Qiang
Yang, Xiao-Dong
Xing, Chun-Gen
author_facet Zhao, Kui
Zhu, Bao-Song
Gong, Wei
Zhu, Mo-Li
Gao, Zhi-Tao
Wu, Yong-You
Chen, Qiang
Yang, Xiao-Dong
Xing, Chun-Gen
author_sort Zhao, Kui
collection PubMed
description INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric cancer of LY294002 in vitro, and we also wanted to know the mechanism of it, which might be applied to clinical tumor therapy. MATERIAL AND METHODS: The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effects of the drugs. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Hoechst 33258 staining was used to detect apoptosis and necrosis morphological changes after LY294002 and/or SN50 treatment. Expression of p53, PUMA and Beclin1 were determined with real-time polymerase chain reaction (RT-PCR) analysis. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after LY294002 and/or SN50 treatment. RESULTS: In this study, we found that treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h, compared to the control group (p < 0.05). Detection of mitochondrial potential and transmission electron microscopic examination indicated that the rate of cell death increased progressively. The expression of p53, PUMA and Beclin1 was up-regulated. CONCLUSIONS: The NF-κB inhibitor SN50 could enhance the role of LY294002 on inducing cell death of human gastric cancer cells SGC7901, which might be a promising new approach to gastric cancer therapy.
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spelling pubmed-39027202014-01-30 SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901 Zhao, Kui Zhu, Bao-Song Gong, Wei Zhu, Mo-Li Gao, Zhi-Tao Wu, Yong-You Chen, Qiang Yang, Xiao-Dong Xing, Chun-Gen Arch Med Sci Basic Research INTRODUCTION: In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric cancer of LY294002 in vitro, and we also wanted to know the mechanism of it, which might be applied to clinical tumor therapy. MATERIAL AND METHODS: The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effects of the drugs. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Hoechst 33258 staining was used to detect apoptosis and necrosis morphological changes after LY294002 and/or SN50 treatment. Expression of p53, PUMA and Beclin1 were determined with real-time polymerase chain reaction (RT-PCR) analysis. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after LY294002 and/or SN50 treatment. RESULTS: In this study, we found that treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of LY294002 on inducing cell death after 24 h, compared to the control group (p < 0.05). Detection of mitochondrial potential and transmission electron microscopic examination indicated that the rate of cell death increased progressively. The expression of p53, PUMA and Beclin1 was up-regulated. CONCLUSIONS: The NF-κB inhibitor SN50 could enhance the role of LY294002 on inducing cell death of human gastric cancer cells SGC7901, which might be a promising new approach to gastric cancer therapy. Termedia Publishing House 2013-12-26 2013-12-30 /pmc/articles/PMC3902720/ /pubmed/24482641 http://dx.doi.org/10.5114/aoms.2013.39790 Text en Copyright © 2013 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Zhao, Kui
Zhu, Bao-Song
Gong, Wei
Zhu, Mo-Li
Gao, Zhi-Tao
Wu, Yong-You
Chen, Qiang
Yang, Xiao-Dong
Xing, Chun-Gen
SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title_full SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title_fullStr SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title_full_unstemmed SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title_short SN50 enhances the effects of LY294002 on cell death induction in gastric cancer cell line SGC7901
title_sort sn50 enhances the effects of ly294002 on cell death induction in gastric cancer cell line sgc7901
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902720/
https://www.ncbi.nlm.nih.gov/pubmed/24482641
http://dx.doi.org/10.5114/aoms.2013.39790
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