The Local Resolution of Cryo-EM Density Maps

Using local sinusoidal features in a standard statistical testing framework, we propose a definition of local resolution for 3D density maps. The resulting algorithm has no free parameters and may be extended to other imaging modalities. Evaluating the local resolution of single particle reconstruct...

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Detalles Bibliográficos
Autores principales: Kucukelbir, Alp, Sigworth, Fred J., Tagare, Hemant D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903095/
https://www.ncbi.nlm.nih.gov/pubmed/24213166
http://dx.doi.org/10.1038/nmeth.2727
Descripción
Sumario:Using local sinusoidal features in a standard statistical testing framework, we propose a definition of local resolution for 3D density maps. The resulting algorithm has no free parameters and may be extended to other imaging modalities. Evaluating the local resolution of single particle reconstructions and sub-tomogram averages shows variable resolution across a 4 to 40Å range.

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