The Local Resolution of Cryo-EM Density Maps

Using local sinusoidal features in a standard statistical testing framework, we propose a definition of local resolution for 3D density maps. The resulting algorithm has no free parameters and may be extended to other imaging modalities. Evaluating the local resolution of single particle reconstruct...

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Detalles Bibliográficos
Autores principales: Kucukelbir, Alp, Sigworth, Fred J., Tagare, Hemant D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903095/
https://www.ncbi.nlm.nih.gov/pubmed/24213166
http://dx.doi.org/10.1038/nmeth.2727
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author Kucukelbir, Alp
Sigworth, Fred J.
Tagare, Hemant D.
author_facet Kucukelbir, Alp
Sigworth, Fred J.
Tagare, Hemant D.
author_sort Kucukelbir, Alp
collection PubMed
description Using local sinusoidal features in a standard statistical testing framework, we propose a definition of local resolution for 3D density maps. The resulting algorithm has no free parameters and may be extended to other imaging modalities. Evaluating the local resolution of single particle reconstructions and sub-tomogram averages shows variable resolution across a 4 to 40Å range.
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spelling pubmed-39030952014-07-01 The Local Resolution of Cryo-EM Density Maps Kucukelbir, Alp Sigworth, Fred J. Tagare, Hemant D. Nat Methods Article Using local sinusoidal features in a standard statistical testing framework, we propose a definition of local resolution for 3D density maps. The resulting algorithm has no free parameters and may be extended to other imaging modalities. Evaluating the local resolution of single particle reconstructions and sub-tomogram averages shows variable resolution across a 4 to 40Å range. 2013-11-10 2014-01 /pmc/articles/PMC3903095/ /pubmed/24213166 http://dx.doi.org/10.1038/nmeth.2727 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kucukelbir, Alp
Sigworth, Fred J.
Tagare, Hemant D.
The Local Resolution of Cryo-EM Density Maps
title The Local Resolution of Cryo-EM Density Maps
title_full The Local Resolution of Cryo-EM Density Maps
title_fullStr The Local Resolution of Cryo-EM Density Maps
title_full_unstemmed The Local Resolution of Cryo-EM Density Maps
title_short The Local Resolution of Cryo-EM Density Maps
title_sort local resolution of cryo-em density maps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903095/
https://www.ncbi.nlm.nih.gov/pubmed/24213166
http://dx.doi.org/10.1038/nmeth.2727
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