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Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities

Bacteria living on the aerial parts of plants (the phyllosphere) are globally abundant and ecologically significant communities and can have significant effects on their plant hosts. Despite their importance, little is known about the ecological processes that drive phyllosphere dynamics. Here, we d...

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Autores principales: Maignien, Loïs, DeForce, Emelia A., Chafee, Meghan E., Eren, A. Murat, Simmons, Sheri L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903271/
https://www.ncbi.nlm.nih.gov/pubmed/24449749
http://dx.doi.org/10.1128/mBio.00682-13
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author Maignien, Loïs
DeForce, Emelia A.
Chafee, Meghan E.
Eren, A. Murat
Simmons, Sheri L.
author_facet Maignien, Loïs
DeForce, Emelia A.
Chafee, Meghan E.
Eren, A. Murat
Simmons, Sheri L.
author_sort Maignien, Loïs
collection PubMed
description Bacteria living on the aerial parts of plants (the phyllosphere) are globally abundant and ecologically significant communities and can have significant effects on their plant hosts. Despite their importance, little is known about the ecological processes that drive phyllosphere dynamics. Here, we describe the development of phyllosphere bacterial communities over time on the model plant Arabidopsis thaliana in a controlled greenhouse environment. We used a large number of replicate plants to identify repeatable dynamics in phyllosphere community assembly and reconstructed assembly history by measuring the composition of the airborne community immigrating to plant leaves. We used more than 260,000 sequences from the v5v6 hypervariable region of the 16S rRNA gene to characterize bacterial community structure on 32 plant and 21 air samples over 73 days. We observed strong, reproducible successional dynamics: phyllosphere communities initially mirrored airborne communities and subsequently converged to a distinct community composition. While the presence or absence of particular taxa in the phyllosphere was conserved across replicates, suggesting strong selection for community composition, the relative abundance of these taxa was highly variable and related to the spatial association of individual plants. Our results suggest that stochastic events in early colonization, coupled with dispersal limitation, generated alternate trajectories of bacterial community assembly within the context of deterministic selection for community membership.
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spelling pubmed-39032712014-01-30 Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities Maignien, Loïs DeForce, Emelia A. Chafee, Meghan E. Eren, A. Murat Simmons, Sheri L. mBio Research Article Bacteria living on the aerial parts of plants (the phyllosphere) are globally abundant and ecologically significant communities and can have significant effects on their plant hosts. Despite their importance, little is known about the ecological processes that drive phyllosphere dynamics. Here, we describe the development of phyllosphere bacterial communities over time on the model plant Arabidopsis thaliana in a controlled greenhouse environment. We used a large number of replicate plants to identify repeatable dynamics in phyllosphere community assembly and reconstructed assembly history by measuring the composition of the airborne community immigrating to plant leaves. We used more than 260,000 sequences from the v5v6 hypervariable region of the 16S rRNA gene to characterize bacterial community structure on 32 plant and 21 air samples over 73 days. We observed strong, reproducible successional dynamics: phyllosphere communities initially mirrored airborne communities and subsequently converged to a distinct community composition. While the presence or absence of particular taxa in the phyllosphere was conserved across replicates, suggesting strong selection for community composition, the relative abundance of these taxa was highly variable and related to the spatial association of individual plants. Our results suggest that stochastic events in early colonization, coupled with dispersal limitation, generated alternate trajectories of bacterial community assembly within the context of deterministic selection for community membership. American Society of Microbiology 2014-01-21 /pmc/articles/PMC3903271/ /pubmed/24449749 http://dx.doi.org/10.1128/mBio.00682-13 Text en Copyright © 2014 Maignien et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Maignien, Loïs
DeForce, Emelia A.
Chafee, Meghan E.
Eren, A. Murat
Simmons, Sheri L.
Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title_full Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title_fullStr Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title_full_unstemmed Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title_short Ecological Succession and Stochastic Variation in the Assembly of Arabidopsis thaliana Phyllosphere Communities
title_sort ecological succession and stochastic variation in the assembly of arabidopsis thaliana phyllosphere communities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903271/
https://www.ncbi.nlm.nih.gov/pubmed/24449749
http://dx.doi.org/10.1128/mBio.00682-13
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