Cargando…
Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior
Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both me...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903281/ https://www.ncbi.nlm.nih.gov/pubmed/24473130 http://dx.doi.org/10.1128/mBio.00972-13 |
_version_ | 1782301077697200128 |
---|---|
author | Lee, Sang Jun Trostel, Andrei Adhya, Sankar |
author_facet | Lee, Sang Jun Trostel, Andrei Adhya, Sankar |
author_sort | Lee, Sang Jun |
collection | PubMed |
description | Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both metabolic network changes far from the d-galactose pathway and changes in the global gene regulatory network. The concentration change of a critical intermediate disturbs the equilibrium state, generating a ripple effect through several metabolic pathways that ends up signaling up- or downregulation of specific sets of genes in a programmed manner to cope with the imbalance. Such long-range effects on metabolites and genetic regulatory mechanisms not only may be a common feature in bacteria but very likely operate during cellular development and differentiation in higher organisms as well as in disease cells, like cancer cells. |
format | Online Article Text |
id | pubmed-3903281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-39032812014-01-30 Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior Lee, Sang Jun Trostel, Andrei Adhya, Sankar mBio Research Article Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both metabolic network changes far from the d-galactose pathway and changes in the global gene regulatory network. The concentration change of a critical intermediate disturbs the equilibrium state, generating a ripple effect through several metabolic pathways that ends up signaling up- or downregulation of specific sets of genes in a programmed manner to cope with the imbalance. Such long-range effects on metabolites and genetic regulatory mechanisms not only may be a common feature in bacteria but very likely operate during cellular development and differentiation in higher organisms as well as in disease cells, like cancer cells. American Society of Microbiology 2014-01-28 /pmc/articles/PMC3903281/ /pubmed/24473130 http://dx.doi.org/10.1128/mBio.00972-13 Text en Copyright © 2014 Lee et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lee, Sang Jun Trostel, Andrei Adhya, Sankar Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title | Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title_full | Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title_fullStr | Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title_full_unstemmed | Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title_short | Metabolite Changes Signal Genetic Regulatory Mechanisms for Robust Cell Behavior |
title_sort | metabolite changes signal genetic regulatory mechanisms for robust cell behavior |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903281/ https://www.ncbi.nlm.nih.gov/pubmed/24473130 http://dx.doi.org/10.1128/mBio.00972-13 |
work_keys_str_mv | AT leesangjun metabolitechangessignalgeneticregulatorymechanismsforrobustcellbehavior AT trostelandrei metabolitechangessignalgeneticregulatorymechanismsforrobustcellbehavior AT adhyasankar metabolitechangessignalgeneticregulatorymechanismsforrobustcellbehavior |