Cargando…

Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach

BACKGROUND: We have previously shown that a candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative subjects, with production of Th2-type cytokines. In addition, such a ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrizzo, Annacarmen, Tagliamonte, Maria, Tornesello, Maria Lina, Buonaguro, Franco M, Buonaguro, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903560/
https://www.ncbi.nlm.nih.gov/pubmed/24428943
http://dx.doi.org/10.1186/1479-5876-12-11
_version_ 1782301111439327232
author Petrizzo, Annacarmen
Tagliamonte, Maria
Tornesello, Maria Lina
Buonaguro, Franco M
Buonaguro, Luigi
author_facet Petrizzo, Annacarmen
Tagliamonte, Maria
Tornesello, Maria Lina
Buonaguro, Franco M
Buonaguro, Luigi
author_sort Petrizzo, Annacarmen
collection PubMed
description BACKGROUND: We have previously shown that a candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative subjects, with production of Th2-type cytokines. In addition, such a candidate idiotype vaccine induces an early gene expression pattern, characterized by the strong induction of an innate immune response, and a late pattern, characterized by a prevalent B cell response. Nonetheless, some HCV-positive individuals showed a complete lack of maturation of circulating APCs with low levels of cytokine production, strongly suggesting the possible identification of selective impairments in immune response in individual subjects. METHOD: Peripheral blood mononuclear cells (PBMCs) were stimulated ex vivo with IGKV3-20 for 24 h and 6 days. Analysis of the global gene expression profile as well as the cytokine pattern was performed for individual subjects. RESULTS: The gene expression profile showed a strong agreement with the cytokine pattern. Indeed, the expression pattern of immune-related genes is highly predictive of the individual immunological phenotype. CONCLUSION: The overall results represent a proof of concept, indicating the efficacy of such an ex vivo screening platform for predicting individual’s responsiveness to an antigen as well as guiding optimization of vaccine design. Larger cohort study will be needed to validate results observed in the study.
format Online
Article
Text
id pubmed-3903560
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-39035602014-01-28 Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach Petrizzo, Annacarmen Tagliamonte, Maria Tornesello, Maria Lina Buonaguro, Franco M Buonaguro, Luigi J Transl Med Research BACKGROUND: We have previously shown that a candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative subjects, with production of Th2-type cytokines. In addition, such a candidate idiotype vaccine induces an early gene expression pattern, characterized by the strong induction of an innate immune response, and a late pattern, characterized by a prevalent B cell response. Nonetheless, some HCV-positive individuals showed a complete lack of maturation of circulating APCs with low levels of cytokine production, strongly suggesting the possible identification of selective impairments in immune response in individual subjects. METHOD: Peripheral blood mononuclear cells (PBMCs) were stimulated ex vivo with IGKV3-20 for 24 h and 6 days. Analysis of the global gene expression profile as well as the cytokine pattern was performed for individual subjects. RESULTS: The gene expression profile showed a strong agreement with the cytokine pattern. Indeed, the expression pattern of immune-related genes is highly predictive of the individual immunological phenotype. CONCLUSION: The overall results represent a proof of concept, indicating the efficacy of such an ex vivo screening platform for predicting individual’s responsiveness to an antigen as well as guiding optimization of vaccine design. Larger cohort study will be needed to validate results observed in the study. BioMed Central 2014-01-15 /pmc/articles/PMC3903560/ /pubmed/24428943 http://dx.doi.org/10.1186/1479-5876-12-11 Text en Copyright © 2014 Petrizzo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Petrizzo, Annacarmen
Tagliamonte, Maria
Tornesello, Maria Lina
Buonaguro, Franco M
Buonaguro, Luigi
Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title_full Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title_fullStr Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title_full_unstemmed Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title_short Prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
title_sort prediction of individual immune responsiveness to a candidate vaccine by a systems vaccinology approach
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903560/
https://www.ncbi.nlm.nih.gov/pubmed/24428943
http://dx.doi.org/10.1186/1479-5876-12-11
work_keys_str_mv AT petrizzoannacarmen predictionofindividualimmuneresponsivenesstoacandidatevaccinebyasystemsvaccinologyapproach
AT tagliamontemaria predictionofindividualimmuneresponsivenesstoacandidatevaccinebyasystemsvaccinologyapproach
AT tornesellomarialina predictionofindividualimmuneresponsivenesstoacandidatevaccinebyasystemsvaccinologyapproach
AT buonagurofrancom predictionofindividualimmuneresponsivenesstoacandidatevaccinebyasystemsvaccinologyapproach
AT buonaguroluigi predictionofindividualimmuneresponsivenesstoacandidatevaccinebyasystemsvaccinologyapproach