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Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution
Protein stability arises from a combination of factors which are often difficult to rationalise. Therefore its improvement is better addressed through directed evolution than by rational design approaches. In this study, five rounds of mutagenesis/recombination followed by high-throughput screening...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903626/ https://www.ncbi.nlm.nih.gov/pubmed/24475252 http://dx.doi.org/10.1371/journal.pone.0087209 |